Tap to "Tap", Pathobiome Associates Health Care

Not Applicable

Completed

• Conditions: Otorhinolaryngologic Diseases
• Interventions: Other: mouth swab

• Registration Number: NCT02751658
• Lead Sponsor: University Hospital, Montpellier

Brief Summary

The bacteria live in community and in some cases it is the combination of several microorganisms that facilitates transmission and pathogenicity. The concept of pathobiome follows from this finding. Investigators hypothesize that the microbial community water point is a pathobiome influencing installation and transmission of pathogens associated with care

Detailed Description

Goals :

Key: To study the impact of bacterial communities on the risk of transmission of waterborne pathogenic bacterium Pseudomonas aeruginosa in patients hospitalized from a contaminated water source by this bacterium.

secondary:

1. Assess the role of communities on installing the water points in P. aeruginosa by comparing contaminated and uncontaminated water sources.

2. To evaluate the effect of the quality of water and the conditions of use of water points network on the composition of bacterial communities associated or not with P. aeruginosa.

Experimental Study conducted from 6 points of water, contaminated deliberately experimental backs onto the project to assess conventional and alternative measures decontamination network and water points

Study Timeline

• Study First Submitted: 2016-03-04
• Study First Submitted QC: 2016-04-21
• Study First Posted: 2016-04-26
• Study Start: 2016-11-08
• Primary Completion: 2017-12-18
• Status Verified: 2019-07
• Study Completion: 2019-07-29
• Last Update Submitted: 2019-07-29
• Last Update Posted: 2019-07-31

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 410

Inclusion Criteria

• Patients in the Otorhinolaryngology department

Exclusion Criteria

• Immunocompromised patients
• Patient Opposition to enter the research protocol

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
patients in the Otorhinolaryngology	mouth swab	patients in the Otorhinolaryngology department realization of a levy into the mouth swab on the day of admission to hospital and the day of release

Primary Outcome Measures

Name	Time	Method
Presence of Pseudomonas aeruginosa	Entrance hospitalization (Day 1)	The presence of Pseudomonas aeruginosa will be made from a buccal swab in all patients admitted to the hospital entrance.; Mark of Pseudomonas aeruginosa will be done with and without trypticase soy broth enrichment and isolation on selective agar cetrimide Pseudomonas aeruginosa . The enrichment will detect a port at very low inoculum knowing that the purpose of the analysis is to detect passing of Pseudomonas aeruginosa from the environment to the patient regardless of the associated infectivity. The identification of certainty will be carried out by MALDI-TOF. If several morphotypes are visualized on agar Cetrimide they will all be identified and kept under custody broth at -20 ° C.

Secondary Outcome Measures

Name	Time	Method
Presence of infection with Pseudomonas aeruginosa	Exit hospitalization (Day 10)	The presence of Pseudomonas aeruginosa will be made from a buccal swab in all patients exit hospitalization.; Mark of Pseudomonas aeruginosa will be done with and without trypticase soy broth enrichment and isolation on selective agar cetrimide Pseudomonas aeruginosa . The enrichment will detect a port at very low inoculum knowing that the purpose of the analysis is to detect passing of Pseudomonas aeruginosa from the environment to the patient regardless of the associated infectivity. The identification of certainty will be carried out by MALDI-TOF. If several morphotypes are visualized on agar Cetrimide they will all be identified and kept under custody broth at -20 ° C.