Pathobiomes Training in the Care of Water Sources and Dynamics of Water-borne Pathogens Involved in Healthcare Associated Infections
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 410
- 主要终点
- Presence of Pseudomonas aeruginosa
研究概览
简要总结
The bacteria live in community and in some cases it is the combination of several microorganisms that facilitates transmission and pathogenicity. The concept of pathobiome follows from this finding. Investigators hypothesize that the microbial community water point is a pathobiome influencing installation and transmission of pathogens associated with care
详细描述
Goals :
Key: To study the impact of bacterial communities on the risk of transmission of waterborne pathogenic bacterium Pseudomonas aeruginosa in patients hospitalized from a contaminated water source by this bacterium.
secondary:
- Assess the role of communities on installing the water points in P. aeruginosa by comparing contaminated and uncontaminated water sources.
- To evaluate the effect of the quality of water and the conditions of use of water points network on the composition of bacterial communities associated or not with P. aeruginosa.
Experimental Study conducted from 6 points of water, contaminated deliberately experimental backs onto the project to assess conventional and alternative measures decontamination network and water points
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 90 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients in the Otorhinolaryngology department
排除标准
- •Immunocompromised patients
- •Patient Opposition to enter the research protocol
研究组 & 干预措施
patients in the Otorhinolaryngology
patients in the Otorhinolaryngology department realization of a levy into the mouth swab on the day of admission to hospital and the day of release
干预措施: mouth swab (Other)
结局指标
主要结局
Presence of Pseudomonas aeruginosa
时间窗: Entrance hospitalization (Day 1)
The presence of Pseudomonas aeruginosa will be made from a buccal swab in all patients admitted to the hospital entrance. Mark of Pseudomonas aeruginosa will be done with and without trypticase soy broth enrichment and isolation on selective agar cetrimide Pseudomonas aeruginosa . The enrichment will detect a port at very low inoculum knowing that the purpose of the analysis is to detect passing of Pseudomonas aeruginosa from the environment to the patient regardless of the associated infectivity. The identification of certainty will be carried out by MALDI-TOF. If several morphotypes are visualized on agar Cetrimide they will all be identified and kept under custody broth at -20 ° C.
次要结局
- Presence of infection with Pseudomonas aeruginosa(Exit hospitalization (Day 10))