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Clinical Trial on The Use of Autologous Bone Marrow Stem Cells in Amyotrophic Lateral Sclerosis (Extension CMN/ELA)

Phase 1
Completed
Conditions
Amyotrophic Lateral Sclerosis
Interventions
Procedure: Intrathecal infusion of autologous bone marrow stem cells
Procedure: Intrathecal infusion of placebo (saline solution).
Procedure: Laminectomy and bone marrow stem cells transplantation
Registration Number
NCT01254539
Lead Sponsor
Fundacion para la Formacion e Investigacion Sanitarias de la Region de Murcia
Brief Summary

The purpose of this clinical trial is to assess the feasibility and the security of the intraspinal and intrathecal infusion of autologous bone marrow stem cells for the treatment of Amyotrophic Lateral Sclerosis patients.

Detailed Description

Patients with Amyotrophic Lateral Sclerosis (ALS) typically endure a progressive paralysis due to the continued loss of motoneurons that leads them to death in less than 5 years. No treatment has changed its natural history. Intraspinal injections of bone marrow mononuclear cells (MNC) have been able to ameliorate the course of ALS in murine models, acting as pumps of trophic factors that keep the motoneurons functional. Moreover, the clinical trial (Study NCT00855400 on www.ClinicalTrials.gov) conducted by our research group to determine the safety and efficacy of Autologous Stem Cell transplantation in Amyotrophic Lateral Sclerosis in humans, found that this procedure is feasible and safe. Continuing with that study, we have designed a phase I/II clinical trial to check the feasibility of the intraspinal and intrathecal infusion of autologous bone marrow stem cells.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
63
Inclusion Criteria
  • Diagnose established following the World Federation of Neurology criteria
  • More than 6 and less than 36 months of evolution of the disease
  • Medullar onset of the disease
  • More than 18 and less than 70 years old
  • Forced Vital Capacity ≥ 50%
  • Total time of oxygen saturation <90% inferior to 5% of the sleeping time
  • Signed informed consent
Exclusion Criteria
  • Neurological or psychiatric concomitant disease
  • Need of parenteral or enteral nutrition through percutaneous endoscopic gastrostomy or nasogastric tube
  • Concomitant systemic disease
  • Treatment with corticosteroids, immunoglobulins or immunosuppressors during the last 12 months
  • Inclusion in other clinical trials
  • Unability to understand the informed consent

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Intrathecal infusion of autologous bone marrow stem cellsIntrathecal infusion of autologous bone marrow stem cellsPatients were drawn 2 ml of cerebrospinal fluid and infused 2 ml (two 1 ml syringes) of Autologous Stem Cells.
Intrathecal infusion of placebo (saline solution).Intrathecal infusion of placebo (saline solution).Patients were infused 2 ml of saline solution
Autologous bone marrow stem cells intraspinal transplantationLaminectomy and bone marrow stem cells transplantationT3-T4 laminectomy and bone marrow ficoll separated mononuclear autologous cells intraspinal transplantation
Primary Outcome Measures
NameTimeMethod
Forced vital capacityEvery 3 months
Secondary Outcome Measures
NameTimeMethod
Absence of adverse eventsEvery week / month depending on the study phase
Neurophysiological variables: Electromyography, polysomnography, evoked potentialsEvery 3 months
Neuroradiological variables: Spinal Magnetic Resonance Imaging (MRI)Every 3 months
Respiratory variables: Maximal inspiratory pressure (PIM), Maximal expiratory pressure (PEM), sniff nasal, oxymetry.Every 3 months
Psychological variables: Health Questionnaire (EuroQol-5D), The Profile of Mood States (POMS)Every 3 months
Neurological variables: The Amyotrophic Lateral Sclerosis Functional Rating Scale (ALS-FRS), Medical Research Council (MRC) and Norris scalesEvery 3 months

Trial Locations

Locations (1)

Hospital Universitario Virgen de la Arrixaca

🇪🇸

El Palmar, Murcia, Spain

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