Investigation and Diagnosis of the Chromosome Variation in Donated/abandoned Blastocyst
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 57
- 试验地点
- 2
- 主要终点
- Blastocyst aneuploidy rate
研究概览
简要总结
Blastocysts derived from patients seeking infertility treatment were generated by in vitro fertilization and embryo culture as previously described, and were evaluated using the Gardner system. As part of the embryo selection process, cells of TE biopsy were collected, and blastocysts were vitrified. The clinical TE biopsies were subjected to whole genome amplification (WGA) with SurePlex reagents (Illumina) followed by NGS-based PGT-A using Illumina's VeriSeq kit (Illumina) on a MiSeq system (Illumina) according to the manufacturer's protocol.
详细描述
Collected 200 donate abandonment embryos (well-developed blastocysts) for research under the National Assisted Reproduction Act of Taiwan.
- Collected 200 donate abandonment embryos: Five to six days after egg retrieval, well-developed embryos (called blastocysts).
- Blastocysts derived from patients seeking infertility treatment were generated.
- Embryos biopsies for PGT-A (by use of NGS platforms from our institute) will be used for the validation of our Lab QC embryo.
- To standardize the operating procedures
- Paper writing.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 50 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •the surplus blastocysts
- •parents consent to donate the embryos
排除标准
- •not agree to participate in this program
- •whose embryo morphology and quality do not meet the technical requirements for genetic testing
研究组 & 干预措施
donated abandonment embryos
Embryos biopsies for PGT-A (by use of NGS platforms from our institute) will be used for the validation of our Lab QC.
干预措施: NGS (Diagnostic Test)
结局指标
主要结局
Blastocyst aneuploidy rate
时间窗: 28 days
anormal Karyotype according to human genome 19 or updated version
次要结局
- Whole genome amplification rate(7 days)