The Effect of Acute Exogenous Oral Ketone Supplementation on Immune Cells Function and Immune Cells Histone Β-hydroxybutyrylation
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 12
- 试验地点
- 2
- 主要终点
- β-hydroxybutyrylation of histone in human immune cells using Western blotting
研究概览
简要总结
To conduct a single-arm pilot study to determine how acute ingestion of an exogenous ketone monoester supplement alters the histone lysine β-hydroxybutyrylation and immune function in healthy human monocytes and lymphocytes.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 19 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Over the age of 19
- •Able to fast overnight
排除标准
- •Being a competitive endurance athlete.
- •Following a ketogenic diet, low-calorie diet, periodic fasting regimen, or regularly consuming ketogenic supplements.
- •Being unable to travel to and from the university
- •Being pregnant.
- •Having been diagnosed with a chronic disorder of glucose or fat metabolism, including type 2 diabetes, chronic pancreatitis, or gallbladder disease
- •Being unable to read or communicate in English.
结局指标
主要结局
β-hydroxybutyrylation of histone in human immune cells using Western blotting
时间窗: Before (fasted state) and 2 hours after the consumption of the exogenous ketone supplement.
The β-hydroxybutyrylation of histones in human monocytes and lymphocytes will be assessed before and 2 hours after the consumption of an exogenous ketone supplement. Protein samples will be collected from the cells, and the levels of histone β-hydroxybutyrylation will be quantified using Western blotting.
次要结局
- Change in beta-hydroxybutyrate concentration(Capillary beta-hydroxybutyrate will be measured before, 30, 60, 90, 120 and 180 minutes after the consumption of exogenous ketone supplement.)
- Change in glucose concentration(Capillary glucose concentration will be measured before, 30, 60, 90, 120, and 180 minutes after the consumption of exogenous ketone supplement.)
- Change in blood pressure(Before, 30, 60, 90, 120 and 180 minutes after the consumption of the exogenous ketone supplement.)
- Change in resting heart rate(Before, 30, 60, 90, 120 and 180 minutes after the consumption of the exogenous ketone supplement.)
- Alteration in immune cell functions(Before (fasted state) and 2 hours after the consumption of the exogenous ketone supplement.)
- Monocytes and lymphocytes Immunophenotyping(Before (fasted state) and 2 hours after the consumption of the exogenous ketone supplement.)
- Gastrointestinal Disturbance(Before, and at 30, 60, 90, 120, and 180 minutes after consuming the exogenous ketone supplement.)
研究者
Jonathan Little
Professor
University of British Columbia