Assessment of Interleukin 8 and 10 Level Changes Before and During Root Canal Treatment for Necrotic Pulp With Apical Periodontitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 22
- 试验地点
- 1
- 主要终点
- To assess changes in IL-8 and IL-10 levels in GCF and periapical fluid and evaluate if they are equivalent and can be used as markers of the inflammatory response before and during RCT.
研究概览
简要总结
The goal of this observational study is to evaluate changes in interleukin 8 (IL-8) and interleukin 10 (IL-10) levels in gingival crevicular fluid (GCF) and periapical fluid during root canal treatment in patients with necrotic pulp and apical periodontitis. The main question it aims to answer is:
How do IL-8 and IL-10 levels in GCF and periapical fluid change over the course of treatment?
Participants will:
Provide samples of GCF and periapical fluid at two different time points during their root canal treatment.
Undergo treatment based on standard clinical protocols for necrotic pulp and apical periodontitis.
详细描述
This observational study aims to investigate changes in biomarkers within gingival crevicular fluid (GCF) and periapical fluid during root canal treatment for patients with necrotic pulp and apical periodontitis. Ethical approval was obtained from the Research Ethics Committee (REC) of the Faculty of Dentistry for Girls at Al-Azhar University (P-EN-23-04). Written informed consent will be obtained from all participants after explaining the study's aims and importance.
Study Design:
Patients requiring root canal treatment at the Endodontic Clinic, Faculty of Dental Medicine for Girls, Al-Azhar University, will be recruited based on inclusion and exclusion criteria.
Inclusion Criteria:
Adults aged 18-45 years. Patients without systemic diseases. Patients diagnosed with necrotic pulp and apical periodontitis in a single-rooted tooth with a single canal.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Inclusion Criteria:
- •Adult patients aged between 18 and 45 years.
- •Patients without any systemic diseases.
- •Patients diagnosed with pulp necrosis and apical periodontitis of a single root with a single canal who are seeking endodontic treatment.
- •Patients who have not previously received endodontic treatment or any related therapeutic procedures.
- •Patients with normal periodontal health.
- •Inclusion Criteria:
- •1. Adult patients aged between 18 and 45 years.
- •Patients without any systemic diseases.
- •Patients diagnosed with pulp necrosis and apical periodontitis of a single root with a single canal who are seeking endodontic treatment.
- •Patients who have not previously received endodontic treatment or any related therapeutic procedures.
- •Patients with normal periodontal health.
排除标准
- •Exclusion Criteria:
- •Patients who refuse to participate in the study.
- •Patients who have been on long-term anti-inflammatory medication,received immunosuppressive chemotherapy or taken antibiotics within the last 3 weeks.
- •Patients with systemic health conditions such as cardiovascular and respiratory diseases, diabetes mellitus, HIV infection, or hepatitis.
- •Unrestorable teeth, pregnant women, and smokers will also be excluded from the study
研究组 & 干预措施
assessment of interleukin 8,10 pre and post
Gingival crevicular fluid sampling Gingival crevicular fluid (GCF) will be collected at two different time points. The collection periods will be defined as follows: before initiating access into the root canal (period 1), and before canal filling (period 2).
Periapical Fluid sample:
Periapical fluid samples will be collected at two different time points. The collection periods will be defined as follows: just after crown access and before root canal filling , by introducing 3 sequential sterile absorbent paper points of a size compatible with the root canal. These paper points will be introduced into the canal until they extend 2 mm beyond the radiographically determined apex.
干预措施: Assessment of Interleukin 8 and 10 Level Changes Before and During Root Canal Treatment for Necrotic Pulp with Apical Periodontitis (Diagnostic Test)
结局指标
主要结局
To assess changes in IL-8 and IL-10 levels in GCF and periapical fluid and evaluate if they are equivalent and can be used as markers of the inflammatory response before and during RCT.
时间窗: The study will collect data at various time points, including baseline (pre-RCT) and during RCT.
次要结局
未报告次要终点
研究者
Esraa Mohamed elsagheer Hassan
Dentist at ministry of health
Faculty of Dental Medicine for Girls