Effects of Consuming Artificial Sweeteners and Sugar on Cerebral and Physiological Responses

Not Applicable

Completed

• Conditions: Normal Subjects

• Registration Number: NCT02853773
• Lead Sponsor: University of Lausanne

Brief Summary

This study aims at investigating the acute influence of artificially sweetened beverages consumption (as compared with sugar-sweetened beverages and water consumption) on brain responses to the viewing of food images, on physiological responses (gut-derived hormones and lipid metabolites) and on the food intake behavior.

Detailed Description

This study is made on a randomized controlled crossover design including three conditions (water, sugar, artificial sweeteners). Measurements will be performed in fasting and fed conditions. The test-meal will comprise either an artificially or sugar-sweetened beverage or water (control). The spatio-temporal brain dynamics to the viewing of food pictures will be assessed by means of electroencephalography (EEG) recordings and electrical neuroimaging analyses. Venous blood and urine will be collected. Blood pressure, cardiac frequency and bio-impedance measurements will be performed. Food intake behavior will be assessed by means of questionnaires and a free-choice buffet. Each tested condition (water, sugar, artificial sweeteners) will be preceded by a run-in period during which the participants' diet will be controlled (2-day isoenergetic diet).

Study Timeline

• Study Start: 2016-02
• Study First Submitted: 2016-02-08
• Status Verified: 2016-07
• Study First Submitted QC: 2016-07-29
• Study First Posted: 2016-08-03
• Primary Completion: 2017-09-29
• Study Completion: 2017-09-29
• Last Update Submitted: 2017-10-24
• Last Update Posted: 2017-10-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 18

Inclusion Criteria

• Healthy
• Caucasian
• Right-handed
• Weak to normal physical activity (< 3hours per week)
• Normal-weight (19<BMI<25 kg/m2)
• Regular consumption of sugar-sweetened beverages (>33cl per week)

Exclusion Criteria

• Consumption of artificially sweetened beverages exceeding 33cl per week
• Diabetes
• Cardiovascular, kidney, hepatic and/or psychological disorders
• Blood pressure at rest > 140/90mmHg
• Body weight < 50kg
• Hemoglobin < 13.5g/dl
• Ferritin < 50microg/l
• Drug consumption
• Alcohol consumption (>10g/day)
• Smoking
• Any particular diet (e.g. vegetarianism), food allergy and/or intolerance
• Body weight gain or loss of >3kg during the last 3 months

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Spatio-temporal brain dynamics	differences observed between 60 min before and 60 min after ingestion of a test meal	Spatio-temporal brain dynamics to the viewing of food will be assessed by means of electroencephalography recordings.

Secondary Outcome Measures

Name	Time	Method
spontaneous food intake	240 min after ingestion of a test meal	Food intake behavior will be assessed by monitoring spontaneous food intake when offered a free-choice buffet.
Changes in concentration of blood triglyceride	-120 min before ingestion of a test meal, and 30, 60, 90, 150, and 210 min after ingestion of a test meal	Blood substrate concentration will be measured in fasting conditions and after ingestion of a test meal
Changes in concentration of blood Fibroblast Growth Factor (FGF)-21	-120 min before ingestion of a test meal, and 30, 60, 90, 150, and 210 min after ingestion of a test meal	Blood substrate concentration will be measured in fasting conditions and after ingestion of a test meal
Changes in concentration of blood insulin	-120 min before ingestion of a test meal, and 30, 60, 90, 150, and 210 min after ingestion of a test meal	Blood substrate concentration will be measured in fasting conditions and after ingestion of a test meal

Trial Locations

Locations (1)

Department of Physiology; 🇨🇭 Lausanne, Vaud, Switzerland