Role of Senescent Cells in Uterine Fibroid Pathogenesis
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 150
- 试验地点
- 1
- 主要终点
- Develop an atlas to comprehensively quantify the rate of senescent cell (SnC) types in fibroid and myometrium tissue samples
研究概览
简要总结
The investigators are evaluating the role of senescent cells in uterine fibroids.
详细描述
Uterine fibroids are prevalent tumors of uterus characterized by excessive fibrotic tissues. Using new cutting-edge computational methods, the investigators have found that small groups of senescent cells in fibroids work in concert with immune cells to produce soluble factors in fibroid tissues to create a feed-forward loop leading to fibrosis. This project seeks to unravel key cell-cell communication networks involving senescent and immune cells in fibroids to develop new treatments for uterine fibroids.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 55 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Subjects must be women above the age of ≥18 - ≤55 years.
- •Women with suspected uterine fibroids.
- •Subject must be or have been sexually active or have had a previous vaginal exam that used a speculum.
- •Subject must be able to understand, read and speak English or Spanish and subsequently, be able to understand what she is consenting to in terms of procedures and use/disclosure of protected health information.
排除标准
- •Unwilling to sign the consent form or complete questionnaire.
- •Pregnancy.
- •Cancer of the uterus
结局指标
主要结局
Develop an atlas to comprehensively quantify the rate of senescent cell (SnC) types in fibroid and myometrium tissue samples
时间窗: 5 years
Perform scRNASeq on human fibroids and control myometrium tissue and apply transfer learning algorithm for SnC identification and phenotyping to identify cell-cell communication patterns in fibroids versus control myometrial tissue using Domino to validate computationally predicted senescent cell types. The computationally predicted immune phenotypes will be validated with flow cytometry.
次要结局
- Rate of inhibition of cellular proliferation and ECM deposition by senolytics and senomorphics in fibroids(5 years)
- Validate the concentration senescence-associated secretory profile (SASP) in cell cultures of senescent cells in uterine fibroids(5 years)