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Cytokine Profiles in Children With Obstructive Sleep Apnea

Not Applicable
Completed
Conditions
Obstructive Sleep Apnea Syndrome
Registration Number
NCT00456573
Lead Sponsor
University of Chicago
Brief Summary

The purpose of this study is to compare the cytokine profile of tonsillar and peripheral blood mononuclear cells in patients with obstructive sleep apnea (OSA) and to compare the blood mononuclear cell cytokine secretion profile between patients with obstructive sleep apnea and controls without apnea.

Detailed Description

We will recruit subjects with OSA about to undergo adenotonsillectomy and collect a blood sample and portion of their tonsils after the induction of general anesthesia. The mononuclear cells will be isolated from both these tissues and stimulated with different markers and cytokines assayed and compared. We will also recruit patients with no symptoms of OSA undergoing unrelated elective surgery and ascertain the absence of OSA by having the parents fill out a sleep questionnaire. We will then collect blood samples from these patients after the induction of anesthesia and examine the cytokine secretion profile and compare it to that of the children with OSA.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
40
Inclusion Criteria
  • OSA documented by polysomnography and clinical symptoms.
  • Patients with no symptoms of OSA and negative sleep questionnaire.
Exclusion Criteria
  • Significant systemic disease except mild asthma

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Primary Outcome Measures
NameTimeMethod
levels of cytokines released from PBMCs of OAS subjects and controlsjust prior to surgery
Secondary Outcome Measures
NameTimeMethod
cytokine level comparison between PBMCs and tonsillar lymphocytes within OAS groupsat time of surgery

Trial Locations

Locations (1)

University of Chicago

🇺🇸

Chicago, Illinois, United States

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