Omics Sequencing of Specimen Derived From Patients With Endometriosis
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Endometriosis
- Sponsor
- Zhujiang Hospital
- Enrollment
- 1000
- Locations
- 1
- Primary Endpoint
- metabolomic sequencing
- Status
- Recruiting
- Last Updated
- 4 years ago
Overview
Brief Summary
Endometriosis is a type of chronic inflammatory gynecological disease, mainly took place in peritoneal cavity. In order to investigate the possible correlation between microbes and endometriosis, the investigators planed to perform 16S rDNA amplicon sequencing, metagenomic sequencing and metabolomic sequencing of paired samples derived from feces, cervical mucus, blood and peritoneal fluid.
Investigators
Eligibility Criteria
Inclusion Criteria
- •The subject who is female.
- •The subject who is infertility or sub-fertile.
- •The subject whose age was between 18-52 years old.
- •The subject who is suspected to have endometrioma.
- •The subject whose menstrual cycle length is between 28-35 days.
- •The subject without the history of abdominal or pelvic surgery.
- •The subject who has dysmenorrhea.
- •The subject who has dispareunia.
- •The subject who was informed the risks and benefits of the experiment and provided written informed consent, and gave their approval.
- •The subject who has chronic pelvic pain and the pain get worse during periods.
Exclusion Criteria
- •The subject who has hernia.
- •The subject who is pregnant.
- •The subject who has malignant tumor.
- •The subject who has Gene Therapy history.
- •The subject who has abdominal or pelvic surgery history.
- •The subject who has peritonitis.
- •The subject who is below 18 years old or over 52 years old.
- •The subject who can not endure laparoscopy.
- •The subject who has cardiovascular disease.
- •The subject who has systemic or regional acute inflammation.
Outcomes
Primary Outcomes
metabolomic sequencing
Time Frame: 2019/01/01-2023/12/31
Compare the metabolites concentration differences of specimens derived from control group and Endometriosis group.
16s amplicon sequencing
Time Frame: 2019/01/01-2023/12/31
Compare the microbiota composition differences of specimens derived from control group and Endometriosis group.
metatranscriptomic sequencing
Time Frame: 2019/01/01-2023/12/31
Compare the RNA concentration differences of specimens derived from control group and Endometriosis group.