Comparing the Effects of Plant Based Protein vs. Whey Protein on Performance and Body Composition of Futsal Players

A randomized, double-blind, controlled trial was performed to investigate health and safety following supplementation with a newly developed plant-based protein blend and to compare the effects of this plant-based protein versus whey protein, after an 8-week supplementation protocol, on body composition and performance. Prior to intervention, volunteers' general health and ability to participate in the study were assessed. The study involved 50 male futsal players currently engaged in a training program and competing. Before engaging the 8-week supplementation protocol, participants underwent measurements of body composition, strength and power, and aerobic performance, as well as a full profile of biochemical health markers, and assessment of food and fluid intake. Evaluations were repeated after 4 weeks, for follow up and to detect potential compliance issues, and after 8 weeks of supplementation. Assessments took place in the morning (starting at 7:00 a.m.) after an overnight fast, except for the performance tests, which were performed following consumption of a standard, meal replacement bar.

Anthropometry and Body Composition - Wearing minimal clothing and no shoes, participants had their weight and height measured on a scale and stadiometer (Seca, Hamburg, Germany), respectively. Body composition, namely bone mineral content, fat mass and fat free mass, were evaluated by dual energy X-ray absorptiometry (DXA) (Horizon Wi, Hologic, Waltham, USA) and skeletal muscle through the evaluation of the muscle thickness of the rectus femoris by ultrasonography (model WED-180 HL, Shenzhen,China). Total body water and its intra and extracellular compartments were estimated from whole body resistance (R) and reactance (Xc), measured through bioelectrical impedance analysis (BIA), using a single frequency device of 50 kHz (BIA-101, RJL/Akern Systems, Firenze, Italy). From the raw R and Xc data, the phase angle was additionally determined, using the Akern Software.

Muscle Strength and Power, and Aerobic Performance - Strength was assessed using the handgrip strength test, as well as the back squat and bench press 1 repetition maximum (1 RM). The handgrip strength test was used to evaluate the maximal isometric force of the muscles of the hand and forearm. Using a portable hand dynamometer (JAMAR, Sammons Preston, Bolingbrook, IL, USA), participants were assessed for both hands alternately, in a stand-up position. The maximal force generated out of 3 attempts was considered for analysis. Additionally, muscle strength was evaluated by 1RM back squat and bench press in a Multipower machine, using a velocity-based training device (Vitruve Encoder, Madrid, Spain). Muscle power was assessed during maximal cycling (Wingate test) and jumping movements. During the Wingate test, participants were instructed to cycle (Monark 894 Peak Bike, Vansbro, Sweden) against a predetermined resistance (7.5% body weight), as fast as possible, for 30 seconds. Vertical jumps, namely the squat jump, countermovement jumps using both legs and single legs, as well as the Abalakov countermovement jump, were performed on a multicomponent force platform (Chronojump Boscosystem, Barcelona, Spain) interfaced with a computer. The best out of 3 attempts was also considered for analysis of this methodology. Aerobic performance was assessed by VO2peak and maximal aerobic speed (MAS), determined using a breath-by-breath gas analyzer (Quark, Cosmed, Italy) in an incremental test performed on a treadmill (Axelero, MEDEN-INMED, Koszalin, Poland). After 3 minutes of warm-up at 5 km/h, participants began the test at 6 km/h and 2% inclination. Each minute, the speed increased 1 km/h until volitional exhaustion. The VO2peak was considered the highest 30 sec average value of VO2 and MAS, the speed at the last completed stage.

All tasks were performed by the participants with direct supervision of sport scientists and/or exercise physiologists, with prior knowledge of the methodologies, and experience handling the equipments and corresponding softwares.

Biochemical Markers - For biochemical health markers, saliva and whole blood, in EDTA tubes, were collected per the standard protocols. . From whole blood, plasma was separated when necessary. Blood measurements included hematocrit, glucose, C-reactive protein (CRP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), and creatinine, using spectrophotometry techniques in an automated equipment (Vario Photometer II DP310, Diglobal Gmbh, Berlin, Germany). Saliva was collected for cortisol evaluation in Enzyme-Linked Immunosorbent Assay (ELISA) commercial kits (Salimetrics, PA, USA).