Comparison of Alternative Embryo Culture Systems
试验速览
- 阶段
- 不适用
- 状态
- 撤回
- 发起方
- 试验地点
- 2
- 主要终点
- Blastulation rate
研究概览
简要总结
This study will compare two alternative embryo culture systems. Embryos from each patient will be randomly assigned to one of two systems. One group will be cultured conventionally in oil-covered droplets in dishes. The other group will be assigned to an enclosed system.
详细描述
Qualifying and consenting IVF patients will have approximately half of their developing embryos cultured in droplets covered with oil. The other approximate half of their embryos will be cultured in small enclosed containers. Embryo assignments will be at random. Regardless of culture system, all embryos will be cultured from the bipronuclear stage to the blastocyst stage in the same (Global) media, without the usual refreshing of media on day 3.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Female age 18 to 40, seeking IVF treatment
- •Ability to understand English (to obtain informed consent)
- •Use of an oocyte donor, sperm donor, gestational carrier, and/or genetic testing (with blastocyst biopsy only) are allowed.
排除标准
- •Diminished ovarian reserve as evidenced by either elevated cycle day 3 follicle stimulating hormone (FSH) level (≥10 IU/L) or low antral follicle count (<8 antral follicles)
- •Fewer than five (5) bipronuclear oocytes
- •Day 3 embryo biopsy for any reason
- •No minors may enroll
结局指标
主要结局
Blastulation rate
时间窗: 5 days (sixth day of embryo development)
Proportion of cultured embryos that form blastocysts.
次要结局
- IVF outcome(7 weeks post-transfer)
- Blastocyst morphology(5 days (sixth day of development))
研究者
Bruce Shapiro M.D.
Medical Director
Fertility Center of Las Vegas