Investigating dermal and epidermal cells in keloid pathogenesis
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- The proposed study is an exploratory study to understand the relation between melanocytes and fibroblasts in keloids. It is not a clinical trial, and hence no success/failure is involved.
研究概览
简要总结
Keloids- benign fibroproliferative tumours that either arise spontaneously or afterinjury - are characterized by hyperproliferative fibroblasts with an increasedtendency to differentiate into myofibroblasts. This deregulation of fibroblastfunction causes excessive deposition of collagen in the dermis leading to a visibleraised scar which may spread beyond the borders of the initial injury. Theprevalence of keloids is significantly higher in individuals with skin ofcolour; around 16 % of people in darkly pigmented populations like AfricanAmericans and Asian Americans are known to be affected with keloids. On theother hand, keloids rarely develop in Caucasians, albinos and individuals with vitiligo(on the depigmented patch), all of whom have a lower pigmentation content.
Sincekeratinocytes, melanocytes and fibroblasts maintain skin homeostasis by closelyinteracting with each other via several autocrine and paracrine signallingpathways, we hypothesised that either melanocytes per se and/or themelanin content therein could influence fibroblast biology, leading to thedevelopment or spread of keloids.
To testthe role of perturbed melanocytes - fibroblasts cross talk in keloidpathogenesis, we propose to carry out the following objectives:
A) Understanding Fibroblast-Melanocyte cross-talk inkeloid pathogenesis:
A.1) Understanding the effect of melanocyte-conditioned media(melanocyte secretome) on fibroblast biology (trans-well co-culture set up)
A.2) Understanding the effect of modulation of melanin content inmelanocytes on the fibroblast biology
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 18.00 Year(s) 至 80.00 Year(s)（—）
- 性别
- All
入选标准
- •Individuals diagnosed with keloid (scar spread beyond original borders and no spontaneous regression within 1 year) Having taken no treatment for keloids before (Treatment naïve) Not on any immunomodulator drugs for other conditions Male and female Above 18 years age .
排除标准
- •ïƒ˜Mixed or unclear diagnosis Pregnant/lactating females Patients who refuse to participate in the study Patients having other diseases like diabetes, or with communicable diseases.
结局指标
主要结局
The proposed study is an exploratory study to understand the relation between melanocytes and fibroblasts in keloids. It is not a clinical trial, and hence no success/failure is involved.
时间窗: 1 year
Understanding the effect of modulation of melanin content in melanocytes on the fibroblast biology
时间窗: 1 year
次要结局
- Understanding the effect of melanocyte-conditioned media (melanocyte secretome) on fibroblast biology (trans-well co-culture set up).(1 year)