Understanding the role of the pigment producing cells in the formation of keloids

Not yet recruiting

Conditions: Other hypertrophic disorders of the skin,

Registration Number: CTRI/2021/12/038951

Lead Sponsor: IADVL Indian Association of Dermatology Venereology and Leprosy

Brief Summary: Keloids- benign fibroproliferative tumours that either arise spontaneously or afterinjury - are characterized by hyperproliferative fibroblasts with an increasedtendency to differentiate into myofibroblasts. This deregulation of fibroblastfunction causes excessive deposition of collagen in the dermis leading to a visibleraised scar which may spread beyond the borders of the initial injury. Theprevalence of keloids is significantly higher in individuals with skin ofcolour; around 16 % of people in darkly pigmented populations like AfricanAmericans and Asian Americans are known to be affected with keloids. On theother hand, keloids rarely develop in Caucasians, albinos and individuals with vitiligo(on the depigmented patch), all of whom have a lower pigmentation content.

Sincekeratinocytes, melanocytes and fibroblasts maintain skin homeostasis by closelyinteracting with each other via several autocrine and paracrine signallingpathways, w***e hypothesised that either melanocytes per se and/or themelanin content therein could influence fibroblast biology, leading to thedevelopment or spread of keloids.***

To testthe role of perturbed melanocytes - fibroblasts cross talk in keloidpathogenesis, we propose to carry out the following **objectives**:

A) Understanding Fibroblast-Melanocyte cross-talk inkeloid pathogenesis:

A.1) Understanding the effect of melanocyte-conditioned media(melanocyte secretome) on fibroblast biology (trans-well co-culture set up)

A.2) Understanding the effect of modulation of melanin content inmelanocytes on the fibroblast biology

Detailed Description: Not available

Recruitment & Eligibility

Status: Not Yet Recruiting

Sex: All

Target Recruitment: 20

Inclusion Criteria

Individuals diagnosed with keloid (scar spread beyond original borders and no spontaneous regression within 1 year) Having taken no treatment for keloids before (Treatment naÃ¯ve) Not on any immunomodulator drugs for other conditions Male and female Above 18 years age .

Exclusion Criteria

ïƒ˜Mixed or unclear diagnosis ïƒ˜Pregnant/lactating females ïƒ˜Patients who refuse to participate in the study ïƒ˜Patients having other diseases like diabetes, or with communicable diseases.

Study & Design

Study Type: Observational

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
The proposed study is an exploratory study to understand the relation between melanocytes and fibroblasts in keloids. It is not a clinical trial, and hence no success/failure is involved.	1 year
Understanding the effect of modulation of melanin content in melanocytes on the fibroblast biology	1 year

Secondary Outcome Measures

Name	Time	Method
Understanding the effect of melanocyte-conditioned media (melanocyte secretome) on fibroblast biology (trans-well co-culture set up).	1 year

Trial Locations

Locations (1): Dr. D.Y. Patil Medical College, Hospital and research centre
🇮🇳
Pune, MAHARASHTRA, India
Dr. D.Y. Patil Medical College, Hospital and research centre
🇮🇳Pune, MAHARASHTRA, India
Dr Aayush Gupta
Principal investigator
9545711211
aayushggupta@gmail.com