Multi-omics Study of Peritoneal Dialysis Effluent to Explore Biomarkers of Peritoneal Fibrosis

This study is a cross-sectional study with no clinical intervention and follow-up.

Patients who met the inclusion criteria were enrolled in the study, and demographic indicators and clinical hematological indicators were collected within 2 weeks, clinical assessment of peritoneal function and other indicators were collected within 4 weeks, abdominal diarrhea effusion exfoliated cells and supernatant were collected within 4 weeks, and some patients were collected for fibrosis assessment by wall peritoneal samples. After the clinical sample was tested, correlation analysis was performed to explore the biomarkers of peritoneal fibrosis.

1. Collection of clinical indicators Relevant information such as demographic indicators, primary renal disease, comorbidities, complications, abdominal dialysis regimen, dialysis age, urine output, ultrafiltration volume, peritonitis history, and concomitant medication were recorded.

After the patients were enrolled in the group, they completed a physical examination (weight, blood pressure, BCM measurement, etc.) within 2 weeks, and collected clinical laboratory indicators including whole blood analysis, hsCRP, NT-proBNP, TNI, blood biochemistry (liver and kidney function, electrolytes, blood glucose, HbA1C, blood lipids, calcium, phosphorus, iPTH, iron, total iron binding capacity, ferritin), mGFR, exudate electrolyte, exudate albumin concentration, etc., exudate CA125, and peritoneal CT peritoneal thickness within 3 months. 2. Clinical assessment of peritoneal function Standard peritoneal balance test was performed to evaluate the peritoneal ultrafiltration function (net ultrafiltration volume after 4 hours of 2.5% glucose dialysis solution) and solute transport rate (D/PCR). 3. Peritoneal dialysis effusion collection and exfoliated cell collection 2L of abdominal translate was collected overnight, cell sediment was collected by centrifugation (1500 rpm, 10 min), RNA was extracted by RNA extraction kit for transcriptome sequencing, and the supernatant of the permeate was cryopreserved to -70 oC for metabolomics determination.

3.1 Exfoliated cell RNA-sequencing