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The Effect of Hyperbaric Oxygen Therapy on Aerobic and Anaerobic Physical Fitness

Not Applicable
Completed
Conditions
Healthy
Athletes
Interventions
Device: Hyperbaric Oxygen Therapy
Device: SHAM treatment
Registration Number
NCT03524989
Lead Sponsor
Assaf-Harofeh Medical Center
Brief Summary

Previous evidence showed hyperbaric oxygen can enhance aerobic and anaerobic performance during the exposure. The effect of continuous exposure of hyperbaric oxygen on performance was never evaluated.

Detailed Description

In recent years, several options for physical performance enhancement by increasing blood oxygen content were explored. One option is increasing the number of red blood cells by blood transfusions. Another option is using the erythropoietin hormone. These methods showed an effective increase of maximal oxygen consumption rate (VO2MAX) by 10%. However, due to their inherent risks, the international sports associations banned their uses. A third option of training in high altitude environments, but this option was never proved to be effective.

Another strategy would be increasing the blood oxygen content by increasing the plasma dissolved oxygen concentration. This would only be possible using hyperbaric oxygen therapy -which includes inhaling 100% oxygen in a pressure higher than the normal atmospheric pressure.

Previous evidence showed hyperbaric oxygen can enhance aerobic and anaerobic performance during the exposure. These studies evaluated the effect during a single hyperbaric oxygen exposure. The effect of continuous exposure of hyperbaric oxygen on performance was never evaluated.

The study is designed as a randomized controlled study aiming to evaluate the therapeutic effects of hyperbaric oxygen therapy (HBOT) on the aerobic and anaerobic performance.

After signing an informed consent form, patients will be randomized into 2 groups: treatment and control group. Patients will be invited for baseline evaluations. All patients would be evaluated 2 times - at baseline and after 2 months of follow up, The evaluation will include physical examination, VO2MAX, muscle biopsy for mitochondrial function, aerobic function measurements, cognitive assessment, brain MRI, brain EEG.

Protocols:

Treatment: Patients will receive 40 daily sessions, 5 days/week, 60 minutes each with 5 minutes air break every 30 minutes, 100% oxygen at 2 atmospheres (ATA).

Control: Patients will receive 40 daily sessions, 5 days/week, 60 minutes each with 5 minutes air break every 30 minutes, 21% oxygen (air) at 1.01 ATA.

Upon completion of the study, control group would be offered to crossover and complete additional 40 sessions of 100% oxygen at 2 ATA. A third evaluation would be performed in case patients will complete the protocol.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
59
Inclusion Criteria
  • 18-25 or 40-50 age
  • Professional performance of aerobic sports for ages 18-25 (at least 5 times a week)
  • Moderate and above performance of aerobic sports for ages 40-50 (at least 4 times a week)
  • no chronic illness
  • no significant musculoskeletal injury in the past 3 months
Exclusion Criteria
  • Debilitating significant musculoskeletal injury
  • Previous hyperbaric oxygen exposure
  • Lung pathology
  • Middle or Inner ear pathology
  • Claustrophobia
  • Chronic illness
  • Smoking
  • Chronic medications

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
TreatmentHyperbaric Oxygen TherapyHyperbaric Oxygen Therapy: 2 months of treatment consisting of 40 daily sessions, 60 minutes of 100% oxygen at pressure of 2 ATA each, five days a week
Control/CrossoverSHAM treatmentSHAM therapy: 2 months of treatment consisting of 40 daily sessions, 60 minutes of 21% oxygen at pressure of 1.01 ATA each, five days a week
Primary Outcome Measures
NameTimeMethod
Change from baseline of exercise VO2 MAX at 2 months and 4 monthsbaseline, at 2 months, 4 months

Using a standard exercise test which includes gas analysis, the change in VO2 maxs will be assessed 2 months and 4 months compared to baseline

Secondary Outcome Measures
NameTimeMethod
Change in forced volume capacity (FVC)baseline, at 2 months, at 4 months

Using a standard spirometry, forced volume capacity (FVC) (liters/second) will be measured

Change from baseline of respiration independent adenosine triphosphate (ATP) production (in muscle biopsybaseline, at 2 months, at 4 months

Using a trucut needle, the gluteus maximus will be sampled in a sterile technique for 1-2mg of muscle tissue. Oroboros O2k high resolution respirometry analyzer will be used (Oxygraph-2k Oroboros Instruments, Innsbruck, Austria). Inpendent adenosine triphosphate (ATP) production (proton leak) (nmol/ml) will be calculated.

Change in maximal vertical jump (meters)baseline, at 2 months, at 4 months

Maximal running speed, maximal vertical jump height will be measured according the standards.

Change in forced expiratory volume at one second (FEV1)baseline, at 2 months, at 4 months

Using a standard spirometry, forced expiratory volume at one second (FEV1) (liters/second) will be measured

Change from baseline of maximal respiratory ratebaseline, at 2 months, at 4 months

Using a trucut needle, the gluteus maximus will be sampled in a sterile technique for 1-2mg of muscle tissue. Oroboros O2k high resolution respirometry analyzer will be used (Oxygraph-2k Oroboros Instruments, Innsbruck, Austria). Maximal respiration rate (nmol/ml) will be calculated.

Change in general cognitive functionbaseline, at 2 months, at 4 months

Patients' cognitive functions will be assessed by Neurotrax computerized cognitive tests to generate the general cognitive index (0-100)

Change in maximal running speed (km/hr)baseline, at 2 months, at 4 months

Maximal running speed will be measured according the standards.

Change from baseline of brain blood perfusion in brain MRIbaseline, at 2 months, at 4 months

imaging will be performed for evaluation of brain changes and angiogenesis process using MRI perfusion and microstructure

Change from baseline of basal respiration ratebaseline, at 2 months, at 4 months

Using a trucut needle, the gluteus maximus will be sampled in a sterile technique for 1-2mg of muscle tissue. Oroboros O2k high resolution respirometry analyzer will be used (Oxygraph-2k Oroboros Instruments, Innsbruck, Austria). Basal respiration rate (nmol/ml) will be measured where mitochondrial reserve capacity and ATP-linked respiration will be calculated.

Change from baseline in body fat/muscle ratiobaseline, at 2 months, at 4 months

Using an electric current weight, the fat % and muscle % content will be measured.

Trial Locations

Locations (1)

Assaf-Harofeh Medical Center

🇮🇱

Ramla, Israel

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