Characterization of Immune Semaphorin in Non Alcoholic Fatty Liver Disease and NASH
- Conditions
- Non Alcoholic Fatty Liver Disease
- Interventions
- Other: Evaluation of the staging of fibrosis liverOther: Determine the expression level of semaphorinOther: Determination of the composition of immunity cells
- Registration Number
- NCT02820285
- Lead Sponsor
- Centre Hospitalier Universitaire de Nice
- Brief Summary
Recent epidemiological studies in France showed a high prevalence of obesity (14.5%) and its strong increase in the last 20 years. Among the many complications associated with obesity, liver complications (steatosis and steatohepatitis \[NASH\]) are among the most common. Semaphorins were described in the early 1990. More than 20 types of these proteins have been reported to date. These proteins were used for neural development. Since many functions have also been described. The semaphorins are involved in numerous physiological or physiopathological processes (cardiac morphogenesis, vascular growth, tumor progression), the regulation of immune cells and liver fibrosis. Preliminary studies have allowed to show that dendritic cells infiltrate adipose tissue and initiate the activation of T cells and inflammation. Immune semaphorin are new players in the regulation of inflammation and immune reactions.
The role of immune semaphorin in regulating inflammation in the two compartments (liver and adipose tissue) could be a crucial step that could lead to more severe liver damage. Its dysregulation could explain NASH injuries. The goal is to identify a new mode of regulation of cellular homeostasis in the fatty liver disease. These factors may serve as diagnostic markers or future therapeutic targets.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 148
- Male and female aged 18-65 years
- Patients with body mass index justifying a surgery for obesity (BMI ≥ 40 kg / m2 or BMI ≥ 35 kg / m2 with comorbidities)
- Consumption of alcohol <20 g / d
- Patients affiliated to a social security insurance
- Patients who signed the informed consent
- Hemochromatosis
- Toxic hepatitis
- Deficiency of alpha-1-antitrypsin
- Wilson's disease
- Liver Autoimmune disease (primary biliary cirrhosis, autoimmune hepatitis)
- Hepatitis B, C
- Drug-induced hepatitis
- Presence of HIV status
- Corticosteroids, amiodarone, valproic acid, tamoxifen, anti-inflammatory drugs, lipid lowering agents, testosterone agonists or beta-adrenergic antagonists, orlistat.
- Pregnant or breastfeeding women
- Incarcerated patients or patient under guardianship
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Control patients Evaluation of the staging of fibrosis liver - Control patients Determine the expression level of semaphorin - Patients with overweight, steatosis and steatohepatitis Determination of the composition of immunity cells - Patients with overweight, steatosis and steatohepatitis Determine the expression level of semaphorin - Morbid obese patients Evaluation of the staging of fibrosis liver - Morbid obese patients Determine the expression level of semaphorin - Morbid obese patients Determination of the composition of immunity cells - Control patients Determination of the composition of immunity cells - Patients with overweight, steatosis and steatohepatitis Evaluation of the staging of fibrosis liver -
- Primary Outcome Measures
Name Time Method Determination of the composition of immunity cells Day 0 The investigators determine the composition of immunity cells by Immunohistochemical and biochemical analyses (Western Blotting)
Evaluation of the staging of liver fibrosis Day 0 The investigators determine the stage of liver fibrosis by histological study of biopsies
Determination of the expression of level of semaphorin Day 0 The investigators determine by technic of Reverse Transcription Polymerase Chain Reaction (RT-PCR) the expression of level of semaphorin
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Service d'Hépato-Gastroentérologie - Hôpital de l'Archet
🇫🇷Nice, France