Comparison of benign and malignant body cavity effusion by automated hematological analyser and flow cytometry
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 50
- 试验地点
- 1
- 主要终点
- 1. Total and differential leukocyte counts in body cavity effusion
研究概览
简要总结
COMPARISON OF BENIGN AND MALIGNANT BODY CAVITY EFFUSION BY AUTOMATED HEMATOLOGICAL ANALYSER AND FLOW CYTOMETRY
Malignancies often impact the serosal cavities (pleural, peritoneal, and pericardial), usually through metastatic spread from sources like the lungs, breasts, female genital tract, or gastrointestinal tract. Flow cytometry is not commonly used for characterizing epithelial and mesothelial cells in diagnostic settings. However, it offers the advantage of being a quick quantitative method applicable to live cells in fresh specimens, preventing fixation artifacts. This technique accurately identifies cytoplasmic, nuclear, and surface antigens. Additionally, multicolour flow cytometry enables the simultaneous assessment of multiple antigens.
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 18.00 Year(s) 至 99.00 Year(s)（—）
- 性别
- All
入选标准
- •All cases of body cavity effusion in individuals aged 18 years or more.
排除标准
- •Cases with less than 2ml or degenerated fluid.
结局指标
主要结局
1. Total and differential leukocyte counts in body cavity effusion
时间窗: Baseline
2. Proportion of cases showing expression of Ep-CAM, CD14 and CD45 in suspicious cases or cases with malignancy
时间窗: Baseline
次要结局
- Association of cytological features((presence of reactive mesothelial cells, atypical mitosis, cytological atypia, presence of)
研究者
Archi Agrawal
Maulana Azad medical college