MedPath

One Step Nucleic Acid Amplification in Endobronchial Ultrasound-guided Needle Aspiration Samples

Conditions
Lung Neoplasms
Registration Number
NCT02157857
Lead Sponsor
University Hospital, Essen
Brief Summary

The aim of our study is to investigate if CK19 mRNA-detection via OSNA can accurately detect lymph node metastases in lung cancer patients using EBUS-TBNA samples.

Detailed Description

Regional lymph nodes are assessed before tumor resection and before initiation of chemo- or radiotherapy. Endobronchial ultrasound-guided needle aspiration (EBUS-TBNA) is a well-established method for mediastinal and hilar lymph node staging \[2, 3\]. Samples obtained via this route are much smaller than surgical lymph node biopsies, yet they are able to provide complete and thorough evaluation, this includes current molecular testing.

One Step Nucleic Acid Amplification (OSNA), a molecular test that utilizes CK 19 mRNA-concentration as a target marker is becoming a rapidly dynamic method in quick detection of sentinel lymph node (SLN) metastases in breast cancer, colon cancer and other cancers.

In lung cancer, the role of SLN is not well established. However, recent publications suggest an accurate and rapid detection of lymph node metastases in NSCLC. However this requires complete lymph node dissection either with mediastinoscopy or by other surgical means such as thoracotomy.

The aim of our study is to investigate if CK19 mRNA-detection via OSNA can accurately detect lymph node metastases in lung cancer patients using EBUS-TBNA samples.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
100
Inclusion Criteria
  • CT-scan suspicious for lung cancer,
  • enlarged mediastinal lymph nodes (>10 mm),
  • EBUS-TBNA to be performed,
  • age: 18-99 years
Exclusion Criteria
  • age: <18 years

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
One step nucleic acid amplification of Cytokeratin 19 mRNA6 months

One step nucleic acid amplification of Cytokeratin 19 mRNA, copy number detection

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Ruhrlandklinik

🇩🇪

Essen, NRW, Germany

Ruhrlandklinik
🇩🇪Essen, NRW, Germany
Filiz Oezkan, MD
Principal Investigator
Kaid Darwiche, MD
Contact
00492014334552
kaid.darwiche@ruhrlandklinik.uk-essen.de

MedPath

Empowering clinical research with data-driven insights and AI-powered tools.

© 2025 MedPath, Inc. All rights reserved.