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Regulatory T Cells for Amyotrophic Lateral Sclerosis

Phase 1
Active, not recruiting
Conditions
Amyotrophic Lateral Sclerosis
Interventions
Other: Excipient
Registration Number
NCT05695521
Lead Sponsor
Cellenkos, Inc.
Brief Summary

Phase 1 Safety Run-in Study of 6 patients followed by Phase 1b Randomized, Double Blind, Placebo Control Trial of CK0803, neurotropic, allogeneic, umbilical cord blood derived T regulatory (Treg) cells in additional 60 patients with Amyotrophic Lateral Sclerosis.

Detailed Description

CK0803, neurotrophic allogenic T regulatory Cells (Treg), utilizes Cellenkos' proprietary CRANE technology to generate disease specific products. The primary objective of the upcoming phase 1 study is to establish safety and tolerability of multiple doses of CK0803 in ALS patients. The goal of the phase 1b study is to extend safety and establish efficacy of CK0803 in ALS using the combined assessment of function and survival (CAFS) that ranks patients' clinical outcomes based on survival time and change in the ALS Functional Rating Scale-Revised (ALSFRS-R) score.

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
66
Inclusion Criteria
  • Ability of the subject or his/her legally authorized representative to provide informed consent.
  • Adult ALS subjects (≥18 years of age)
  • Diagnosis of ALS, according to the Revised El Escorial Criteria for ALS
  • Subjects with disease onset ≤ 5 years
  • Upright (sitting position) Slow Vital Capacity (SVC) as adjusted for sex, age and height ≥ 50% predicted
  • Subjects must have documented ALSFRSR score of 36-45 at baseline.
  • Subjects taking concomitant Riluzole or Edaravone or Albrioza at study entry must be on a stable dose for ≥ 30 days prior to the first dose of study treatment (Day 1).
  • Screening values of coagulation parameters including platelet count, international normalized ratio (INR), prothrombin time (PT), and activated partial thromboplastin time (APTT) should be within normal ranges.
  • Agree to practice highly effective contraception during the study and continue contraception for 90 days after their last dose of study treatment.
Exclusion Criteria
  • Uncontrolled infection, not responding to appropriate antimicrobial agents after seven days of therapy. The Protocol medical monitor is the final arbiter of eligibility.
  • Antiplatelet or anticoagulant therapy within the 14 days prior to Day 1 or anticipated use during the study, including but not limited to daily aspirin including low dose aspirin (defined as ≤ 150 mg/day), clopidogrel, dipyridamole, warfarin, dabigatran, rivaroxaban and apixaban
  • Clinically significant low platelet count (defined as < 100,000/mm3), coagulation tests, or laboratory abnormalities that would render a subject unsuitable for inclusion
  • Unwillingness to comply with study procedures, including follow-up, as specified by this protocol, or unwillingness to cooperate fully with the Investigator
  • Have any other conditions, which, in the opinion of the Investigator would make the subject unsuitable for inclusion, or could interfere with the subject participating in or completing the study
  • Concurrent participation in any other interventional clinical study
  • Treatment with another investigational drug, biological agent, or device, including, but not limited to sodium phenylbutyrate, within 1 month of Screening, or 5 half-lives of investigational agent, whichever is longer
  • Treatment of cancer in the last 5 years (except in situ carcinoma of the cervix or basal cell carcinoma)
  • Female subjects who are pregnant or currently breastfeeding
  • Other unspecified reasons that, in the opinion of the Investigator or Sponsor, make the subject unsuitable for enrollment.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboExcipientExcipient
CK0803CK0803CK0803 (cryopreserved, allogeneic, cord blood derived T regulatory cells that express neurotropic homing markers) will be administered intravenously Dose: 100 million Treg cells (fixed dose) Dose regimen: * Induction: one infusion every 7 days (+/-3) x 4 doses * Consolidation: one infusion every 28 days (+/-3) x 5 doses
Primary Outcome Measures
NameTimeMethod
Combined assessment of function and survival (CAFS)24 weeks

CAFS ranks patients' clinical outcomes based on survival time and change in the ALS Functional Rating Scale-Revised (ALSFRS-R) score where score = 0 is worst and score = 48 is best. Each patient's outcome is compared to every other patient's outcome, assigned a score, and the summed scores are ranked. The mean rank score for each treatment group can then be calculated. A higher mean CAFS score indicates a better group outcome.

Treatment Limiting Toxicity (TLT)28 days

TLT of CK0803 as assessed by the incidence and severity of AE and SAEs using NCI-CTCAE Version 5.0 criteria. TLT is a primary endpoint for bothe phase 1 safety run-in and phase 1b RCT part

Secondary Outcome Measures
NameTimeMethod
Slow Vital Capacity (SVC)Each measured at baseline and at weeks 5, 8, 12 and/or 13, 16, 20, 24 and/or 25, 36 and 48 from first infusion and/or at EOT

SVC is the volume of air expired, on a low complete expiration after a maximal inspiration without forced or rapid effort.

Nfl CSFEach measured at baseline and at weeks 5, 8, 12 and/or 13, 16, 20, 24 and/or 25, 36 and 48 from first infusion and/or at EOT

Neurofilament light chain level in the CSF

Nfl SerumEach measured at baseline and at weeks 5, 8, 12 and/or 13, 16, 20, 24 and/or 25, 36 and 48 from first infusion and/or at EOT12 months

Neurofilament light chain level in the Serum

Incidence of all cause AEs and SAEsbaseline and at weeks 1, 2, 3, 4, 5, 8, 12 and/or 13, 16, 20, 24 and/or 25, 36 and 48 from first infusion and/or at end of treatment (EOT)

Treatment limiting toxicities of CK0803 as assessed by the incidence and severity of AE and SAEs using NCI-CTCAE Version 5.0 criteria.

ALS Functional Rating Scale-Revised (ALSFRS-R) ScoreEach measured at baseline and at weeks 5, 8, 12 and/or 13, 16, 20, 24 and/or 25, 36 and 48 from first infusion and/or at EOT

Longitudinal processes of ALSFRS-R score measured at baseline and different time points. ALSFRS-R score 0=worst; 48=best

Ventilation assistance-free survival (VAFS)Each measured at baseline and at weeks 5, 8, 12 and/or 13, 16, 20, 24 and/or 25, 36 and 48 from first infusion and/or at EOT

VAFS is defined as the time to the earliest occurrence of 1 of the following events: i) Death, or ii) Permanent ventilation (\> 22 hours of mechanical ventilation \[invasive or non-invasive\] per day for \> 21 consecutive days in the absence of an acute potentially reversible event)

Overall Survival (OS)24 weeks and 48 weeks from first infusion and/or at EOT

OS defined as the length of time from the start of treatment that patients are still alive.

Handheld dynamometer (HHD)Each measured at baseline and at weeks 5, 8, 12 and/or 13, 16, 20, 24 and/or 25, 36 and 48 from first infusion and/or at EOT

HHD allows for objective measurement of muscle strength

Amyotrophic Lateral Sclerosis Specific Quality of Life - Revised (ALSSQOL-R)Each measured at baseline and at weeks 5, 8, 12 and/or 13, 16, 20, 24 and/or 25, 36 and 48 from first infusion and/or at EOT

Each item of the ALSSQOL-R is rated by the individual using a 0 to 10 point Likert scale, with 0 being the least desirable situation, and 10 being the most desirable.

Trial Locations

Locations (3)

Baylor College of Medicine

🇺🇸

Houston, Texas, United States

Michael E. DeBakey Veterans Affairs Medical Center

🇺🇸

Houston, Texas, United States

Columbia University Irving Medical Center

🇺🇸

New York, New York, United States

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