Influence of a single bout of endurance exercise on epigenetic modifications, effector function and tumorinfiltration of natural killer cells
Not Applicable
- Conditions
- Prostate cancerC61Malignant neoplasm of prostate
- Registration Number
- DRKS00010442
- Lead Sponsor
- Deutsche Sporthochschule Köln Institut für Kreislaufforschung ung Sportmedizin, Abteilung für molekulare und zelluläre Sportmedizin
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete
- Sex
- Male
- Target Recruitment
- 24
Inclusion Criteria
Patients with diagnosed high-risc prostate cancer (PSA > 20ng/ml or biopsie gleason score 8-10 or clinical cT3 stadium); imminent resection of tumor tissue; age > 18 years
Exclusion Criteria
Neoadjuvant chemotherapy or radiation; prior chemotherapy because of prior cancer disease; thrombocytopenia < 20.000/µl; cardivascular diseases
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Primary Endpoint is the change of epigenetic modifications (promoter methylation) of the NKG2D gene due to the intervention. Before, after and 12 h after the single bout of exercise (IG) or rest (CG) blood samples will be drawn. Lymphocytes are attained by centrifugation with a separation medium and are used for a magnetbead isolation of NK cells (natural killer cells). DNA is extracted from NK cells and the promoter methylation of the NKG2D gene is analyzed by bisulfite treatment and real time PCR.
- Secondary Outcome Measures
Name Time Method Besides the primary endpoint also (1) changes of global histone acetylation in NK cells will be investigated. Therefore the cells will be stained by immunocytochemistry for histone acetylation at H4K5 and H3K9. Further (2) the NK cell cytotoxicity will be analyzed by performing cytotoxicity assays with fresh isolated NK cells and the tumor cell line K652. For this purpose the NK cells will also be characterized for their subgroups (CD56bright and CD56dim) by flow cytometry. In addition the resected tumor tissue will be under investigation for (3) the number of intratumoral NK cells and (4) the expression of NKG2D ligands on tumor cells. Both parameters will be analyzed by immunohistochemistry and evaluated microscopically.