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CALIBER Phase 1: A Pilot Study in Normal-weight and Overweight Adults.

Not Applicable
Completed
Conditions
Cardiometabolic Risk
Interventions
Other: Low-carbohydrate, high-fat
Other: High-carbohydrate, moderate-fat
Registration Number
NCT03257085
Lead Sponsor
Liverpool John Moores University
Brief Summary

Pilot study to compare the impact of following a low-carbohydrate, high-fat diet versus following a high-carbohydrates, moderate-fat diet (UK dietary guidelines) on cardiometabolic risk markers and associated behaviours in a normal-weight and overweight adult population.

Detailed Description

Cardiometabolic diseases (CMD), such as type 2 diabetes and cardiovascular disease (CVD), are globally amongst the highest contributors to morbidity and mortality with high (cost) implications to the overall economy and health care systems. A number of risk markers have been associated with CMDs, including blood serum markers, low levels of lean mass and high levels of body fat, including increased waist circumference. Dietary factors and nutritional status have long been linked with specific markers of cardiometabolic (CM) risk. The quantity and quality of dietary carbohydrates has been associated with increased serum triglycerides levels, increased body fat mass, increased waist circumference and visceral fat around the organs in particular. They also seem to increase food cravings. Whilst official dietary guidelines in the UK and elsewhere still recommend a high carbohydrate and low fat diet as standard, these recommendations have increasingly been challenged. Evidence has been mounting that very-low carbohydrate (ketogenic) and low carbohydrate diets can ameliorate CM risk factors, especially when a personalised rather than a one-size-fits-all approach is being taken. Response to carbohydrate load and adherence to dietary interventions can vary widely dependent on individual substrate and energy metabolism and insulin-resistant status.

The majority of dietary interventions with ketogenic and low-carbohydrate diets has focused on weight loss as the primary outcome in overweight and obese individuals. However, in recent years evidence has been mounting that the location and quality of adipose tissue (AT) play a more important role in manifestation of CM risk than quantity of AT alone. Detrimental health behaviours, such as low-quality diet and low levels of physical activity seem to be important contributors to this.

Further studies can provide vital insights into the links between diet, location-specific adipose tissue, CM risk factors and health-related behaviours.

Therefore this 8-weeks randomised pilot study will investigate the impact of either following a low-carbohydrate, high-fat diet versus following a high-carbohydrates, moderate-fat diet (UK dietary guidelines) on cardiometabolic risk markers and associated behaviours in a normal-weight and overweight adult population aged 19 - 64 at potential risk of CMD.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
15
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Low-carbohydrate, high-fatLow-carbohydrate, high-fatParticipants adhering to low-carbohydrate, high-fat diet for 8 weeks.
High-carbohydrate, moderate fatHigh-carbohydrate, moderate-fatParticipants following high-carbohydrate, moderate-fat diet for 8 weeks.
Primary Outcome Measures
NameTimeMethod
Body composition - Bioelectrical impedance8 weeks

Lean mass, fat mass and adipose tissue location and distribution

Tumor necrosis factor alpha and Interleukin 68 weeks

Measured in μg/mL

Serum lipid profile8 weeks

Total cholesterol, HDL-C, LDL-C, non-HDL cholesterol, small-dense LDL-C and triglycerides measured in mmol/L

Blood glucose8 weeks

Measured in mmol/L

Inflammatory markers, such as CRP8 weeks

Measured in mg/L

Adiponectin8 weeks

Measured in μg/mL

Body composition - Anthropometrics8 weeks

Waist, hip, neck, thigh and calf circumference measured in cm

Systolic and diastolic blood pressure8 weeks

Measured in mmHg

Fibroblast growth factor 21 (FGF21).8 weeks

Measured in pg/mL

Secondary Outcome Measures
NameTimeMethod
Food cravings8 weeks

Measured via self-reported questionnaire

Cognition8 weeks

Assessed via self-reported questionnaire

Adherence to fibre recommendations8 weeks

Measured via structured questionnaires

Impact on physical activity patterns8 weeks

Assessed via accelerometry

Clinical traditional and emerging markers of dietary intake8 weeks

Fibroblast growth factor 21 and serum metabolites

Satiety8 weeks

Assessed via serum leptin levels (ng/mL)

Adherence to dietary guidelines8 weeks

Measured via diet quality score assessment

Adherence to assigned diet8 weeks

Measured via 4-day food diaries

Adherence to low-carbohydrate diet8 weeks

Measured via blood ketones (mmol/L)

Experience with either low-carbohydrate, high-fat or high-carbohydrate, moderate-fat diet8 weeks

Assessed via semi-structured interview

Adherence to taking dietary supplement for low-carbohydrate, high-fat group8 weeks

Measured via counting of number of multi-vitamin and mineral supplements consumed

Trial Locations

Locations (1)

Liverpool John Moores University

🇬🇧

Liverpool, Merseyside, United Kingdom

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