Determinants of liver fat composition in overweight and obese humans

Completed

Conditions: fatty liver
Non-alcoholic fatty liver (NAFL)
10019654
10018424

Registration Number: NL-OMON45290

Lead Sponsor: niversiteit Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 19

Inclusion Criteria

Signed informed consent
Caucasian (people will be excluded when having a 50% or more then 50% racial African/Asian background)
Male or postmenopausal female
Aged 45-70 years at start of the study
Body mass index (BMI) 27 * 35 kg/m2
Stable dietary habits (no weight loss or gain >3kg in the past 3 months)
Sedentary lifestyle (not more than 2 hours of sports per week)

Exclusion Criteria

Type 2 diabetes
Active diseases (cardiovascular, diabetes, liver, kidney, cancer or other)
Contra-indication for MRI
Alcohol consumption of >2 servings per day
Smoking >5 cigarettes per day
Use of anti-coagulants

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>- %SFA expressed as relative amount of SFA to the total amount of fatty<br /><br>acids.<br /><br>- DNL expressed as percentage of palmitate in very-low-density-lipoprotein<br /><br>(VLDL)-TG originating from DNL </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- Liver fat composition expressed as relative amount of MUFA and PUFA to the<br /><br>total amount of fatty acids, in addition to %SFA<br /><br>- Adipose tissue fat composition expressed as relative amount of SFA, MUFA and<br /><br>PUFA to the total amount of fatty acids<br /><br>- Adipose tissue fat composition expressed as relative amount of linoleic acid,<br /><br>docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) to the total amount<br /><br>of fatty acids.<br /><br>- Hepatic, peripheral and whole body insulin sensitivity expressed as %<br /><br>suppression of endogenous glucose production (EGP), rate of disappearance (Rd)<br /><br>in mol/kg/min and glucose infusion rate (GIR) in mol/kg/min.<br /><br>- Muscle insulin sensitivity expressed as determinants/markers for muscle<br /><br>insulin sensitivity in muscle biopsy (oxphos, GLUT4, intramyocellular lipids<br /><br>(IMCL).</p><br>