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Safety And Tolerability Of Subcutaneous MANP In Difficult To Control/Resistant Hypertensive Subjects

Phase 1
Completed
Conditions
Resistant Hypertension
Difficult to Control Hypertension
Interventions
Drug: Placebo
Registration Number
NCT05247528
Lead Sponsor
E-Star BioTech, LLC
Brief Summary

A randomized, double-blind, placebo-controlled multiple ascending dose study in hypertensive subjects on stable doses of at least three hypertensive drugs for at least 6 weeks prior to Screening. The study will consist of screening, PK-unit admittance, and safety follow up periods.

Subjects will be randomized at a 6:2 ratio of either MANP or placebo and will be stratified by race in each dosage cohort. The entire first Cohort will be given the lowest dosage with subsequent cohorts progressing sequentially to the higher doses depending on safety and tolerability of the previous cohort.

Endpoints not related to the safety reviews will be analyzed after the last patient last visit (LPLV).

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
37
Inclusion Criteria
  • DTC/RH diagnosed with clinic SBP ≥140 mmHg or DBP ≥ 90 mmHg (or SBP ≥ 130 mmHg or DBP ≥ 80 mmHg for diabetics) while on at least three standard-of-care antihypertensive medications (which must include a diuretic).
  • MDRD eGFR ≥ 30 mL/min.
  • Men and women between the ages of 18 - 80.
  • BMI within the range of 18-40 kg/m2.
  • Women of childbearing potential must not be pregnant and agree to avoid becoming pregnant while receiving study treatment and for 14 days after the last study visit.
Exclusion Criteria
  • HbA1c ≥ 8% at Screening.
  • Use of other investigational drugs within 30 days of screening or foreseen use during the study.
  • Inability to comply with study requirements as judged by the Investigator.
  • Pregnant and/or breastfeeding.
  • Any disease or condition (medical or surgical) which, in the opinion of the investigator, might compromise the hematologic, cardiovascular, pulmonary, renal, gastrointestinal, hepatic, or central nervous system; or other conditions that may interfere with the absorption, distribution, metabolism or excretion of study drug, or would place the subject at increased risk.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
MANPMANP* Subjects randomized to the experimental arm, will receive a single subcutaneous injection each morning daily. (multiple ascending dose cohorts) * Intervention: Drug: MANP
Placebo ComparatorPlacebo* Subjects randomized to the placebo arm, will receive a single subcutaneous injection each morning daily. * Intervention: Placebo
Primary Outcome Measures
NameTimeMethod
Chemistry: BilirubinFrom baseline to Day 21

Change in Total bilirubin (mg/dL)

Chemistry: GlucoseFrom baseline to Day 21

Change in Glucose (mg/dL)

Chemistry: CreatinineFrom baseline to Day 21

Change in Creatinine (mg/dL)

Blood PressureFrom baseline to Days 1-6, Day 12, and 21.

Change in SBP and DBP

Physical Examination: Body PartsFrom baseline to Day 21

Change in investigator assessment of the condition of body parts (head, throat, abdomen, and extremities)

Chemistry: SodiumFrom baseline to Day 21

Change in Sodium concentration.

Chemistry: ChlorideFrom baseline to Day 21

Change in Chloride concentration

Chemistry: Aspartate aminotransferaseFrom baseline to Day 21

Change in AST (IU/L)

Chemistry: Blood urea nitrogenFrom baseline to Day 21

Change in BUN (mg/dL)

Adverse Eventsthrough study completion, an average of 2 months.

Number and percent of participants with one or more Treatment Emergent Adverse Events (TEAEs) or any serious adverse events (SAEs).

Hematology: HemoglobinFrom baseline to Day 21

Change in g/dL Hemoglobin

Hematology: PlateletsFrom baseline to Day 21

Change in platelet count

Chemistry: PotassiumFrom baseline to Day 21

Change in Potassium concentration.

Chemistry: Alanine aminotransferaseFrom baseline to Day 21

Change in ALT (IU/L)

TemperatureFrom baseline to Days 1-6, Day 12, and 21.

Change in Temperature

Hematology: Mean Corpuscular HemoglobinFrom baseline to Day 21

Change in the mean corpuscular hemoglobin.

Hematology: RBCFrom baseline to Day 21

Change in red blood cell count

ECG: QT intervalFrom baseline to Days 1-6 and Day 21.

Change in 12-Lead ECG QT Interval

Chemistry: BicarbonateFrom baseline to Day 21

Change in Bicarbonate concentration

Chemistry: HbA1cFrom baseline to Day 21

Change in percent HbA1c

ECG: QTc intervalFrom baseline to Days 1-6 and Day 21

Change in 12-Lead ECG QTc (Fridericia's) Interval

Hematology HematocritFrom baseline to Day 21.

Change in Percent Hematocrit

Pulse RateFrom baseline to Days 1-6, Day 12, and 21.

Change in Pulse Rate

Hematology: RBC distributionFrom baseline to Day 21

Change in red blood cell distribution width

Hematology: WBCFrom baseline to Day 21

Change in white blood cell count

Chemistry: Alkaline phosphataseFrom baseline to Day 21

Change in Alkaline phosphatase (IU/L)

Physical Examination: OrgansFrom baseline to Day 21

Change in investigator assessment of the condition of organs (skin, eyes, ears, nose, thyroid, lungs, liver, spleen, and lymph nodes)

Abbreviated Neurological ExaminationFrom baseline to Day 21

Change in investigator assessment of neurological condition

Secondary Outcome Measures
NameTimeMethod
MANPFrom baseline for Day 1 & Day 5

Change in amount of Plasma MANP both in plasma.

Cardiac Metrics:From Baseline for Day 1-6

Heart Rate

Cardiac Metrics: Blood pressuresFrom Baseline for Day 1-6

Change in SBP and DBP

MetabolicsFrom baseline for Day 1 & Day 5

Change in amount of non-esterified insulin and glucose.

Serum creatinineFrom Baseline for Day 1-6

Change in Serum creatinine

Urine flow rateFrom Baseline for Day 1-6

Change in urine flow rate

Immune ResponseFrom baseline for Day 1, Day 5, Day 12, & Day 21

Change in amount of anti-MANP and anti-ANP

Urinary Sodium ExcretionFrom Baseline for Day 1-6

Change in amount of urinary sodium excretion

eGFR,From Baseline for Day 1-6

Change in eGFR,

cGMPfrom baseline for Day 1 & Day 5

Change in amount of cGMP both in plasma and urine

Aldosteronefrom baseline for Day 1 & Day 5

Change in amount of aldosterone both in plasma and urine

Trial Locations

Locations (5)

USA Clinical Site 01

🇺🇸

Tustin, California, United States

USA Clinical Site 04

🇺🇸

Decatur, Georgia, United States

USA Clinical Site 05

🇺🇸

Owensboro, Kentucky, United States

USA Clinical Site 03

🇺🇸

Anniston, Alabama, United States

USA Clinical Site 02

🇺🇸

DeLand, Florida, United States

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