Comparison of Biofilm Characteristics Between an in Situ Biofilm Model of Supragingival Plaque and Natural Teeth
Overview
- Phase
- Early Phase 1
- Intervention
- Not specified
- Conditions
- Biofilm
- Sponsor
- University of Santiago de Compostela
- Enrollment
- 15
- Locations
- 1
- Primary Endpoint
- Bacterial viability
- Last Updated
- 9 years ago
Overview
Brief Summary
The purpose of this study is to determine in situ the bacterial viability, thickness and bacterial diversity of a biofilm formed on different substrates in comparison to a naturally tooth-formed oral biofilm. In addition, it will be evaluated whether the restraint of oral hygiene measures may influence the development of the PL-Biofilm.
Detailed Description
* To calculate an a priori sample size, the following statistical criteria were established: an effect size of 0.35, an alpha error of 0.05 and a statistical power of 87%. Assuming these criteria and using the repeated measures ANOVA test, a sample size of 15 subjects was required. * Bacterial viability and thickness will be analyse by Confocal Laser Scanning Microscope and Live/Dead BackLight staining solution. * Bacterial diversity will be analyse by Pyrosequencing the 16S DNAr gene.
Investigators
Inmaculada Tomas
Senior Lecture
University of Santiago de Compostela
Eligibility Criteria
Inclusion Criteria
- •systemically healthy
- •good oral health status
Exclusion Criteria
- •smoker or former smoker,
- •presence of dental prostheses or orthodontic devices,
- •antibiotic treatment or routine use of oral antiseptics in the previous 3 months,
- •presence of any systemic disease that could alter the production or composition of saliva
Outcomes
Primary Outcomes
Bacterial viability
Time Frame: Bacterial viability measured in proportion of viable/no viable bacteria after 48 hours of exposition to oral cavity
It will be measured by analysis of images taken by Confocal laser Scanning Microscope
Bacterial diversity
Time Frame: Bacterial diversity measured by microbial estimators of abundance and richness after 48 hours of exposition to oral cavity
It will be measured by 16S rDNA gene pyrosequencing and posterior bioinformatic analysis.
Secondary Outcomes
- Biofilm thickness(Oral biofilm thickness measured by analysis of sectional images after 48 hours of exposition to oral cavity)