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Safety, Tolerability, and Efficacy of Saroglitazar Mg 4 mg in Liver Transplant Recipients With Nonalcoholic Fatty Liver Disease (NAFLD)

Phase 2
Completed
Conditions
Liver Transplant; Complications
NAFLD
Interventions
Registration Number
NCT03639623
Lead Sponsor
Zydus Therapeutics Inc.
Brief Summary

This is a phase 2A, single center, open-label, single-arm, 24-week study to evaluate the safety, tolerability and efficacy of Saroglitazar Magnesium 4 mg in liver transplant recipients with NAFLD.

Detailed Description

This is a phase 2A, single center, open-label, single-arm, 24-week study to evaluate the safety, tolerability and efficacy of Saroglitazar Magnesium 4 mg in liver transplant recipients with NAFLD.

The study will be conducted over a period of up to 33 weeks and will include 5 weeks screening, a 24 week treatment period and 4 week follow-up period. The primary end point of the study is to assess the safety of Saroglitazar Magnesium 4 mg in liver transplant recipients with NAFLD over 24 weeks of treatment.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
20
Inclusion Criteria
  • Able and willing to give written informed consent.
  • Males or females, 18 to 75 years of age.
  • Patients who are at least 6 months post-transplant for nonalcoholic steatohepatitis (NASH) or cryptogenic cirrhosis thought to be secondary to NASH are eligible for enrolment.
  • The presence of NAFLD determined by Magnetic resonance imaging-derived proton density fat fraction (MRI-PDFF) prior to enrollment.
  • Patients with ≤20% variance in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin between Visit 1 and Visit 1.1.
  • History of medical compliance with immunosuppression.
  • Female subjects of non-child bearing potential or on highly effective contraception. For male subjects with female partners of childbearing potential, willing to follow highly effective contraception measures during the study, either by the male participant or his female partner or both.
Exclusion Criteria
  • Pregnant or lactating females.

  • Patient with abnormal transaminases due to secondary intercurrent illness.

  • Patients with bile duct strictures.

  • Other causes of chronic liver disease after liver transplantation including autoimmune, viral, and alcoholic liver disease.

  • Graft cirrhosis as defined by:

    1. Cirrhosis on historical liver biopsy.
    2. Evidence of cirrhosis on imaging including portal venous collaterals.
    3. Prior history of decompensated liver disease including ascites, hepatic encephalopathy or variceal bleeding.
    4. Evidence of esophageal varices on prior endoscopy.
  • Body mass index (BMI) <18 kg/m².

  • Subjects with change in body weight >5% in the 3 months prior to enrollment.

  • Subjects requiring corticosteroid or anticoagulation therapy.

  • History of myopathies or evidence of active muscle diseases.

  • Unstable cardiovascular disease.

  • History of bladder disease and/or hematuria or has current hematuria unless due to a urinary tract infection.

  • Active malignancy post-liver transplantation.

  • History of malignancy in the past 5 years and/or active neoplasm.

  • History of chronic rejection of liver transplant graft.

  • Acute cellular rejection of liver transplant graft within the past 6 months.

  • Evidence of Acute cellular rejection (ACR) or chronic rejection (CR) or alternative etiologies to NAFLD.

  • Poorly controlled diabetes as defined by an HbA1c >8.5% within the past 6 months.

  • History of excessive alcohol intake.

  • Subject tests positive for a urine drug screen.

  • Subject has a history of chronic (uncontrolled) pain.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Saroglitazar Magnesium 4 mgSaroglitazarSaroglitazar Magnesium tablet once daily in the morning 60 minutes before breakfast
Primary Outcome Measures
NameTimeMethod
Number of Participants With Adverse Events Assessed by CTCAE24 weeks

Safety measured by adverse events, vital signs, physical exams, body weight, electrocardiograms (ECGs) and lab results (including hematology, chemistry and urinalysis)

Secondary Outcome Measures
NameTimeMethod
Very Low-density Lipoprotein (Atherogenic Lipoprotein)Baseline and Week 24

Changes in subtypes of very low-density lipoprotein (VLDL) from baseline to EOT

Very Low-density Lipoprotein Chylomicron Triglyceride (Atherogenic Lipoprotein)Baseline and Week 24

Changes in VLDL chylomicron triglyceride from baseline to EOT

Hepatic FatBaseline and week 24

Changes in hepatic fat as determined by MRI-PDFF from baseline to end-of-treatment (EOT)

Liver StiffnessBaseline and Week 24

Changes in Liver stiffness as determined by MRE from baseline to end-of-treatment (EOT) Liver stiffness is a measure of a mechanical property of tissue (stiffness). This can be measured non-invasively by MR elastography, a technique that involves applying an external vibration to the abdomen and measuring the progression of shear waves through the underlying liver using MRI.

Frequently Sampled Intravenous Glucose Tolerance Test (Insulin Resistance Marker)Baseline and Week 24

Changes in frequently sampled intravenous glucose tolerance test (FSIVGTT) from baseline to EOT

Glycosylated Hemoglobin (Insulin Resistance Marker)Baseline and Week 24

Changes in glycosylated hemoglobin (HbA1c) from baseline to EOT

Fructosamine (Insulin Resistance Marker)Baseline and Week 24

Changes in fructosamine from baseline to EOT

Serum Liver EnzymesBaseline and Week 24

Changes in serum liver enzymes from baseline to EOT

Serum Liver Enzymes; BilirubinBaseline and Week 24

Changes in bilirubin from baseline to EOT

Serum LipidsBaseline and Week 24

Changes in serum lipids from baseline to EOT

Small Dense Low-density Lipoprotein (Atherogenic Lipoprotein)Baseline and Week 24

Changes in small dense low-density lipoprotein (sdLDL) from baseline to EOT

LDL Size (Atherogenic Lipoprotein)Baseline and Week 24

Changes in LDL size from baseline to EOT

LDL Concentration (Atherogenic Lipoprotein)Baseline and Week 24

Changes in LDL concentration from baseline to EOT

High-density Lipoprotein (Atherogenic Lipoprotein)Baseline and Week 24

Changes in high-density lipoprotein (HDL) from baseline to EOT

Change in Metabolic Flexibility; Time to Peak RQBaseline and Week 24

Metabolic flexibility was measured via a whole room calorimeter (i.e. respiration chamber). The CO2 production and O2 consumption were recorded every minute for a total of 18 hours while study participants were in the respiration chamber on baseline and Week 24. Metabolic flexibility was quantified by measuring whole-body CO2 production relative to O2 consumption or respiratory quotient (RQ). The RQ oscillates between 0.7 and 1.0, which is indicative of either predominantly fatty acid or carbohydrate oxidation, respectively.

The time to maximal carbohydrate consumption is measured by the time it takes to reach peak RQ after a standardized meal, whereas maximal carbohydrate consumption is measured at the peak of RQ vs. time curve. Next, the transition from maximal carbohydrate consumption to maximal fatty acid consumption is measured from peak RQ to lowest RQ. Finally, fasting biofuel utilization is measured in the fasting state and represents predominantly fatty acid oxidation.

Quality of Life (SF-36 Health Survey)Baseline and Week 24

Change in Quality of life score from baseline to EOT The SF-36 is a multi-purpose, short-form health survey with 36 questions. It yields an 8-scale profile of functional health and well-being scores (domains) as well as psychometrically based physical and mental health summary measures. The SF-36 taps 8 health concepts: physical functioning, bodily pain, physical role functioning, emotional role functioning, emotional well-being, social functioning, vitality, and general health perceptions. The 8 scales are further summarized to 2 distinct higher-ordered clusters: the PCS and mental composite t-score (MCS). The range for all 8 domains as well as for the composite t-scores is from 0 to 100 with 100 as best possible health status and 0 as the worst health status

Peak Plasma Concentration [Cmax]PK Sampling time points: Day 1 and Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar following first and last dose.

Time to Reach Peak Plasma Concentration [Tmax]PK Sampling time points: Day 1 and Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar following first and last dose

Area Under Plasma Concentration vs. Time Curve Till the Last Time Point [AUC0-t]PK Sampling time points: Day 1 visit at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Area Under Plasma Concentration vs. Time Curve Extrapolated to the Infinity [AUC0-∞]PK Sampling time points: Day 1 visit at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Area Under Plasma Concentration vs. Time Curve in a 24 h Dosing Interval [AUCtau]PK Sampling time points: Day 1 and Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Elimination Rate Constant [λz]PK Sampling time points: Day 1 and Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Elimination Half-life [t1/2]PK Sampling time points: Day 1 and Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Apparent Volume of Distribution [Vd/F]PK Sampling time points: Day 1 (Baseline) and Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Apparent Clearance [CL/F]PK Sampling time points: Day 1 and Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Minimal or Trough Plasma Concentration [Cmin]PK Sampling time points: Week 24 visit at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Accumulation Index Calculated as a Ratio of AUCtau (Last Dose)/AUCtau (First Dose)PK Sampling time points: Day 1 and Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Pharmacokinetics of Saroglitazar

Fluctuation IndexPK Sampling time points: Week 24 visits at pre-dose (0), 0.5, 1.0, 2, 3, 4, 6, 8, 10 and 24-hour post-dose

Fluctuation index is the peak trough fluctuation within complete dosing interval at steady state, calculated as PTF (%) = (\[Cmax - Cmin\]/Cav ) multiplied by 100

Trial Locations

Locations (1)

Virginia Commonwealth University

🇺🇸

Richmond, Virginia, United States

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