Gene Polymorphism of IL17 and IL 23

Completed

• Conditions: COVID-19 Respiratory Infection
• Interventions: Diagnostic Test: laboratory test

• Registration Number: NCT04948515
• Lead Sponsor: Sohag University

Brief Summary

rates are in such considerable amount among large population base. The pathogenesis of COVID-19 implicates a potent inflammatory response, involving a complex group of mediators including interleukins. These pleiotropic cytokines are secreted at the region of inflammation and released into the circulation by a variety of different cell types, including macrophages, lymphocytes, endothelial cells, epithelial cells during sepsis and acute organ injuries. There has recently found a number of studies reporting that the cytokine storms take part in the course of COVID-19

Detailed Description

Aim Of The Study

1. To evaluate if there is an association between the IL-17, IL-23 gene polymorphism in Patients With COVID 19 by real-time PCR

2. To measure the serum level of IL17 by ELISA in COVID 19 patients by ELISA.

3. To detect if there is an association between gene polymorphism of IL17 and IL23 and severity of a condition in patients with COVID 19 Study design: a cross-sectional study.

The blood samples will be collected from patient records in COVID19 isolation hospitals in sohag governorate and isolation department in sohag university hospitals.

Study Timeline

• Study Start: 2021-06-03
• Study First Submitted: 2021-06-24
• Study First Submitted QC: 2021-06-24
• Study First Posted: 2021-07-02
• Primary Completion: 2021-11-30
• Study Completion: 2021-12-03
• Status Verified: 2022-10
• Last Update Submitted: 2022-10-24
• Last Update Posted: 2022-10-25

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 120

Inclusion Criteria

• Patients with Covid19 positive PCR

Exclusion Criteria

• Patients with Covid19 clinical criteria not confirmed by PCR

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
group 2 patients with non severe covid 19 respiratory infections	laboratory test	Patients with nonsevere covid 19 admitted to the internal ward, blood samples will be collected for testing different genotypes and serum level of IL 17
group 1 patients with severe covid 19 respiratory infections	laboratory test	Patients with severe covid 19 admitted to ICU, blood samples will be collected for testing different genotypes and serum level of IL 17

Primary Outcome Measures

Name	Time	Method
genotyping of IL 23	3-6-2021 to 30-8-2021	real time pcr detection of different genotypes
genotyping of IL 17	3-6-2021 to 30-8-2021	real time pcr detection of different genotypes
estimation of serum level of IL 17	3-6-2021 to 30-8-2021	measuring serum level of IL 17 by ELISA

Trial Locations

Locations (1)

Sohag University; 🇪🇬 Sohag, Egypt