Modification of the Expression of ADARs and PDE8A Editing in Suicidal Behavior
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 600
- 试验地点
- 1
- 主要终点
- Evolution of the modification of the expression of Adenosine deaminases acting on RNA (ADARs) and of the editing profile of phospho-diesterase 8A (PDE8A)
研究概览
简要总结
Suicidal behavior (SB) is a major public health problem in France, with more than 10,000 suicides and 220,000 suicide attempts per year.
According to the commonly accepted model for understanding suicidal behavior, individuals who carry a suicidal act when subjected to stress factors (environmental stress, depression, substance ...) are those which have a specific vulnerability.
These vulnerabilities can be considered as clinical parameters (propensity to despair, aggressive and/or impulsive traits), neurobiological parameters (dysfunction of the serotonergic system, ...) and cognitive parameters (taking disadvantageous decision ...). Suicidal vulnerability is partly underpinned by genetic factors. The interest of current researches is to identify biomarkers that will improve the opportunities for early identification of subject with a risk for SB. Numerous scientific studies, including post-mortem studies of the brains of suicide completers, have established a link between dysregulation of the ribonucleic acids editing (RNA) of certain genes, the enzymatic activity of Adenosine deaminases acting on RNA (ADARS) responsible for this edition and suicidal behavior. A prospective study is needed to quantify and qualify in the blood of depressed patients (with or without a history of suicide) and healthy controls, the editing changes and the expression and alteration of the activity of ADARS.
详细描述
Over two years, 600 participants will be recruited:
- 225 subjects with current major depressive episode and an history of suicide attempt (depressed suicide attempters)
- 225 subjects with current major depressive episode but with no personal history of suicide attempt (affective controls)
- 150 subjects with no history of psychopathology whole life (healthy controls)
Each patient will attend a total of 3visits during a follow-up period of 6 months +/- 15 days (inclusion, visit at 3 and 6 months).
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •No specific inclusion criteria :
- •18 to 65 years
- •Subject who signed the informed consent
- •Able to understand the nature, purpose and methodology of the study
- •Able to understand and perform the clinical and neuropsychological evaluations.
- •Specific inclusion criteria depressed suicide attempters:
- •Subject whose primary psychiatric diagnosis is a major depressive episode according to Diagnostic and Statistical Manual of Mental Disorders -5 (DSM-5) criteria
- •Personal history of suicide attempt
- •affective controls:
- •Subject whose primary psychiatric diagnosis is a major depressive episode according to DSM-5 criteria
- •No personal history of suicide attempt
- •healthy controls:
- •No personal history of psychiatric disorders (Axis I ) defined by the Mini International Neuropsychiatric Interview (MINI) according to the DSM-5 criteria
- •No history of suicide attempt
排除标准
- •Refusal of participation
- •Deprived of liberty Subject (by judicial or administrative decision)
- •Subject protected by law (guardianship)
- •Subject exclusion period in relation to another protocol
- •Subject is not affiliated to a social security scheme, beneficiary or not such a plan
- •Subject for which the maximum annual amount of allowances of € 4,500 has been reached
- •Pregnant women
- •Breastfeeding Women
研究组 & 干预措施
Blood sample for genetic purpose
All the participants performed the same evaluations and blood analysis.
The study is composed of 3 groups :
- depressed patients with an history of suicide attempt
- depressed patients without any history of suicide attempt
- healthy controls without any history of psychopathology
干预措施: Blood sample for genetic purpose (Other)
结局指标
主要结局
Evolution of the modification of the expression of Adenosine deaminases acting on RNA (ADARs) and of the editing profile of phospho-diesterase 8A (PDE8A)
时间窗: At the inclusion visit, 3 months and 6 months after the inclusion
Studying ADARs expression and RNA editing of genes associated with SB, including PDE8A and comparison of these results between healthy controls and depressed patients with or without history of SB
次要结局
- Modification of the expression and RNA editing of Cluster of differentiation 24 (CD24)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Three prime repair exonuclease 1 (TREX1)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Interferon stimulated gene 15 (ISG15)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression of ADAR1a enzymes(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Spindle And Kinetochore Associated protein 2 (SKA2)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression of ADAR1b enzymes(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression of ADAR2 enzymes(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Spermidine/Spermine N1-Acetyltransferase 1 (SAT1)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Interleukins (ILs)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Chemokines (CXCLs)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Brain derived Neurotrphic factor (BDNF)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Tumor necrosis factor alpha (TNF alpha)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Vascular endothelial growth factor (VEGF)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of Hydroxytryptamine receptor 2A (HTR2A)(At the inclusion visit, 3 months and 6 months after the inclusion)
- Modification of the expression and RNA editing of insulin-like growth factor protein 7 (IGFB7)(At the inclusion visit, 3 months and 6 months after the inclusion)