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Single Oral Dose of BeneFlax to Healthy Young and Older Adults

Phase 2
Completed
Conditions
Diabetes Mellitus, Type 2
Hypercholesterolemia
Interventions
Other: BeneFlax - 38% secoisolariciresinol diglucoside (SDG)
Registration Number
NCT01531569
Lead Sponsor
University of Saskatchewan
Brief Summary

This study is being done to look at age differences in the way the investigators bodies break down a compound found in flax seed (secoisolariciresinol diglucoside or SDG). It is administered to research subjects in a product called BeneFlax, which a concentrated version of flax seed containing 38% SDG.

It is known that as people age, their bodies undergo physical changes both on the outside and the inside. The aging process may change the way that the investigators bodies deal with compounds the investigators eat. As an important measure of safety, the investigators want to check for evidence whether there is a difference in break down of SDG between different age groups.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
22
Inclusion Criteria
  • Healthy adults: 18-45 and 60-80 years of age
Exclusion Criteria
  • Strict vegetarians and vegans (as these diets likely contain foods which have higher levels of lignans)
  • Strict vegetarians and vegans (as these diets likely contain foods which have higher levels of lignans)
  • Individuals who smoke
  • Individuals who have experienced diarrhea in the last three months
  • Individuals who have taken oral antibiotics in the last three months
  • Individuals who are currently taking warfarin or any of its derivatives
  • Individuals with low haemoglobin (<121g/L for women and <137g/L for men)
  • Individuals with BMI under 19 or over 28
  • Pregnant or lactating women
  • Women with child bearing potential not using contraceptives
  • Current diagnosis of a bleeding disorder or at risk of bleeding
  • Individuals with gastrointestinal problems (such as ulcers), or convulsive, depressive, or hepatic disorders
  • Individuals with diabetes mellitus
  • Individuals currently taking a flax seed supplement
  • Individuals with an allergy to flax seed
  • Individuals who have donated blood or lost > 450 mL of blood within 56 days of study duration
  • Individuals who have participated in any other clinical trial with and investigational agent within one month of starting this trial

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
BeneFlaxBeneFlax - 38% secoisolariciresinol diglucoside (SDG)BeneFlax given as a single oral dose to assess pharmacokinetics
Primary Outcome Measures
NameTimeMethod
Time to reach peak plasma concentration (tmax) of secoisolariciresinol, enterodiol and enterolactone.0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 72, 96 hours post-dose

Blood samples will be collected at baseline and then post-dosing: every three hours for the first 48 hours, once at 72 hours and once at 96 hours. The concentrations of secoisolariciresinol, enterodiol and enterolactone will be quantitated at each time point.

Peak plasma concentration (Cmax) of secoisolariciresinol, enterodiol and enterolactone.0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 72, 96 hours post-dose

Blood samples will be collected at baseline and then post-dosing: every three hours for the first 48 hours, once at 72 hours and once at 96 hours. The concentrations of secoisolariciresinol, enterodiol and enterolactone will be quantitated at each time point.

Terminal phase half-life of secoisolariciresinol, enterodiol and enterolactone.0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 72, 96 hours post-dose

Blood samples will be collected at baseline and then post-dosing: every three hours for the first 48 hours, once at 72 hours and once at 96 hours. The concentrations of secoisolariciresinol, enterodiol and enterolactone will be quantitated at each time point.

Area under the plasma concentration versus time curve (AUC) of secoisolariciresinol, enterodiol and enterolactone.0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 72, 96 hours post-dose

Blood samples will be collected at baseline and then post-dosing: every three hours for the first 48 hours, once at 72 hours and once at 96 hours. The concentrations of secoisolariciresinol, enterodiol and enterolactone will be quantitated at each time point.

Elimination rate constant (k) of secoisolariciresinol, enterodiol and enterolactone.0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 72, 96 hours post-dose

Blood samples will be collected at baseline and then post-dosing: every three hours for the first 48 hours, once at 72 hours and once at 96 hours. The concentrations of secoisolariciresinol, enterodiol and enterolactone will be quantitated at each time point.

Secondary Outcome Measures
NameTimeMethod
Inflammatory markersat 0 hours - prior to study commencement

Measurement of the inflammatory markers interleukin-1a, interleukin-1b, interleukin-6 and TNF-a to determine participants baseline levels. The levels will only be tested once prior to study commencement

Activity questionnaireat 0 hours - prior to study commencement

Background information about participants usual physical activities will be collected once prior to study commencement.

Food frequency questionnaireat 0 hours - prior to study commencement

Background information about participants usual dietary choices will be collected once prior to study commencement.

Trial Locations

Locations (1)

Saskatoon Centre for Patient Oriented Research - Saskatoon City Hospital

🇨🇦

Saskatoon, Saskatchewan, Canada

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