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Dietary Impact on Intestinal Sulfate Metabolism

Not Applicable
Completed
Conditions
Healthy Nutrition
Interventions
Behavioral: Animal-based diet then plant-based diet
Behavioral: Plant-Based Diet then animal-based diet
Registration Number
NCT05333900
Lead Sponsor
University of Minnesota
Brief Summary

This is a 5-week crossover pilot study of 15 highly motivated healthy volunteers who will consume two 7-day intervention diets: 1) a diet low in sulfur-containing amino acids (Low-S diet), emphasizing plant-based foods and fat sources; and 2) a diet high in sulfur-containing amino acids (High-S diet), emphasizing animal protein and fat sources. A 14-day washout period will follow each intervention, in which participants will eat their typical diets.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
15
Inclusion Criteria
  • Provide informed consent
  • Ambulatory and community dwelling
  • Able and willing to comply with the study schedule and procedures
  • 18 - 80 years of age
  • BMI between 18.5 - 40.0 kg/m2
  • Omnivorous or willing to consume animal products through duration of study
Exclusion Criteria
  • Antibiotic use within 3 months
  • Planned antibiotic therapy within the period of the study, e.g., perioperative antibiotics.
  • Use of sulfonamides or sulfasalazine, as these sulfur-containing medications may confound results
  • Use of 5-aminosalicylates (5-ASA), as they are known to inhibit sulfidogenesis
  • History of anaphylactic food allergies, e.g., peanuts, seafood.
  • Food intolerances and allergies, including gluten sensitivity, lactose intolerance, and intolerance of high fiber dietary content.
  • Strict vegan eating practices, with refusal to consume animal products.
  • Planned use of oral probiotics while on study.
  • Serious, concomitant illness that, in the opinion of the Investigator, would interfere with evaluation of safety or efficacy, or put the participant at risk of harm from study participation.
  • Significant alcohol use, defined as > 20 g/day in females and > 30 g/day in males for a period of 3 months within one year prior to screening.
  • Underlying chronic gastrointestinal disease that can cause diarrhea, including short bowel syndrome, irritable bowel syndrome, malabsorption, and celiac disease.
  • History of partial or complete colectomy.
  • History of malabsorptive bariatric surgery.
  • Currently participating in another clinical study.
  • Legally incompetent and unable to understand the study's purpose, significance and consequences, and to make decisions accordingly.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Animal-based then plant basedAnimal-based diet then plant-based dietplant based: diet low in sulfur-containing amino acids (Low-S diet), emphasizing plant-based foods and fat sources animal based: A diet high in sulfur-containing amino acids (High-S diet), emphasizing animal protein and fat sources
Plant-based then animal basedPlant-Based Diet then animal-based dietplant based: diet low in sulfur-containing amino acids (Low-S diet), emphasizing plant-based foods and fat sources animal based: A diet high in sulfur-containing amino acids (High-S diet), emphasizing animal protein and fat sources
Primary Outcome Measures
NameTimeMethod
Effect of Short-term (1-week) Plant- and Animal-based Eating Patterns on Hydrogen Sulfide ProductionFollowing 1-week of plant- and animal-based eating patterns

Determine the effect of short-term (1-week) plant- and animal-based eating patterns on ex vivo fecal hydrogen sulfide production in healthy human volunteers We will measure fecal hydrogen sulfide production ex vivo in ppm/g of dry weight of stool

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

University of Minnesota

🇺🇸

Minneapolis, Minnesota, United States

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