utrition-based approach to support antigen-specific immunotherapy in subjects with cow's milk or peanut allergy
Completed
- Conditions
- Food Allergy10001708
- Registration Number
- NL-OMON43691
- Lead Sponsor
- Dermatologie/Allergologie
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 30
Inclusion Criteria
Patients with established cow*s milk or peanut allergy (determined by food provocation) in the age of 18-65 years from our outpatient clinic Allergology/Dermatology
Exclusion Criteria
Pregnancy and use of systemic immunosuppressants (the continuous use, not local usage as an effect of an allergic reaction, e.g. prednison)
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The T-cell response (measured by cytokine production) and phenotype of these<br /><br>cells (Th1/Th2/Treg by flow cytometry) in patients before and after stimulation<br /><br>of their isolated cells from blood with specific allergen (whey or peanut) and<br /><br>the influence and effect of NDOs on this response (with/without presence of<br /><br>artificial CpG DNA or Bifidobacterium breve DNA)</p><br>
- Secondary Outcome Measures
Name Time Method <p>Changes in cytokine and chemokine profile of PBMCs after stimulation with NDOs<br /><br>will be studied. Also changes in cytokine and proliferation within<br /><br>subpopulations of the PBMCs will be studied (e.g. Dendritic cells or T-cells)<br /><br>and the interaction between these groups. To study safety of the NDOs, the<br /><br>activation of basophils will also be monitored.</p><br>