The Effect of Vitamin C Supplementation on Gut Microbiota Composition and Metabolic Activity in Healthy Adults
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 23
- 试验地点
- 1
- 主要终点
- Butyrate (Short Chain Fatty Acid)
研究概览
简要总结
The aim of this dietary intervention study is to explore how vitamin C affects the bacteria that live in our gut. Vitamins are essential nutrients found in fruits and vegetables. Our bodies cannot make them on their own, but we need them to function correctly. Vitamins play various roles, including supporting the immune system and assisting with energy production. Some vitamins in our diet can reach the large intestine, where they may be used by gut bacteria to promote their growth. In this study, we aim to investigate how our gut bacteria interact with vitamin C and how this interaction affects their growth and activity.
For this study, participants will follow their habitual diet for one-week (run-in period), followed by two consecutive two-week supplementation periods in which they will first take a moderate dose (200 mg/day) and then a high-dose (1000 mg/day) of vitamin C. A final one-week period follow up period will involve a return to their habitual diet. Faecal, blood and urine samples will be collected at the start and end of each supplementation period to explore changes in gut microbiota composition, activity and markers of inflammation.
详细描述
This is a sequential dietary intervention trial exploring the effects of two doses of vitamin C supplementation on gut microbiota: a moderate, diet-achievable dose of 200 mg/day, and a high dose of 1000 mg/day, each given for two weeks. Primary outcomes will be gut microbiota activity (SCFA production) and composition while secondary exploratory outcomes will include systemic inflammation and gut barrier integrity markers. We anticipate that this pilot study will provide valuable insights into the dose-response effects of vitamin C and help define optimal intakes for promoting gut health.
Twenty-three healthy adults will be recruited from the Glasgow area, with all study visits taking place at the New Lister Building, University of Glasgow. Each participant will attend four study visits over six weeks.
The intervention includes:
- One-week run-in period (habitual diet)
- Two weeks of moderate-dose vitamin C (Vitamin C Chewable Tablet 200 mg, one tablet per day)
- Two weeks of high-dose vitamin C (Vitamin C Chewable Tablet 1000 mg, one tablet per day)
- One-week follow-up (habitual diet)
There will be no washout period between the study periods and participants will be instructed to maintain their usual diet and lifestyle throughout the trial.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Sequential
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy individuals aged 18-65 years with a BMI between 18.5-35 Kg/m2
- •Self-reported good health with no chronic conditions requiring regular medical care
- •Willing to provide blood, urine, and stool samples at multiple time points
排除标准
- •Aged <18 or >65 years
- •Chronic illness requiring regular medication or GP visits
- •Current or recent medication affecting gut transit or digestion
- •Major gastrointestinal surgery
- •Pregnant or breastfeeding
- •Regular use of pre/probiotics, vitamins, or minerals (unless willing to discontinue 2-4 weeks prior)
- •Antibiotics in past 3 months
- •Weight change >±2 kg in past month
- •Participation in other research likely to interfere with this study
研究组 & 干预措施
Sequential vitamin C intervention
In this single-arm, sequential dietary intervention study, participants will follow their normal habitual diet for one week (run-in period) and then receive two different doses of vitamin C tablets over two consecutive 14-day periods: a moderate dose of 200 mg/day during the first period (2 weeks) and a high dose of 1000 mg/day during the second period (another 2 weeks). The study also includes one week post intervention follow up period (habitual diet) after the high vitamin C dose where participants return to their habitual diet without vitamin C supplementation
干预措施: Moderate Vitamin C dose (200 mg) (Dietary Supplement)
Sequential vitamin C intervention
In this single-arm, sequential dietary intervention study, participants will follow their normal habitual diet for one week (run-in period) and then receive two different doses of vitamin C tablets over two consecutive 14-day periods: a moderate dose of 200 mg/day during the first period (2 weeks) and a high dose of 1000 mg/day during the second period (another 2 weeks). The study also includes one week post intervention follow up period (habitual diet) after the high vitamin C dose where participants return to their habitual diet without vitamin C supplementation
干预措施: High-dose vitamin C supplementation (1000 mg) (Dietary Supplement)
Sequential vitamin C intervention
In this single-arm, sequential dietary intervention study, participants will follow their normal habitual diet for one week (run-in period) and then receive two different doses of vitamin C tablets over two consecutive 14-day periods: a moderate dose of 200 mg/day during the first period (2 weeks) and a high dose of 1000 mg/day during the second period (another 2 weeks). The study also includes one week post intervention follow up period (habitual diet) after the high vitamin C dose where participants return to their habitual diet without vitamin C supplementation
干预措施: Habitual diet (run in period) (Other)
结局指标
主要结局
Butyrate (Short Chain Fatty Acid)
时间窗: Baseline (Visit 1), Week 1 (Visit 2), Week 3 (Visit 3), Week 5 (Visit 4) and Week 6 (Visit 5)
Quantified in stool samples using Gas Chromatography
Short Chain Fatty Acids i.e. acetate, propionate, butyrate, total
时间窗: Baseline (Visit 1), Week 1 (Visit 2), Week 3 (Visit 3), Week 5 (Visit 4) and Week 6 (Visit 5)
Quantified in stool samples using Gas Chromatography
Stool microbiota composition analysis
时间窗: Baseline (Visit 1), Week 1 (Visit 2), Week 3 (Visit 3), Week 5 (Visit 4) and Week 6 (Visit 5)
16S rRNA gene amplicon sequencing from faecal DNA extracts
次要结局
- Stool pH(Baseline (Visit 1), Week 1 (Visit 2), Week 3 (Visit 3), Week 5 (Visit 4) and Week 6 (Visit 5))
- Vitamin C in plasma(Week 1 (Visit 2), Week 3 (Visit 3), and Week 5 (Visit 4))
- Inflammatory markers(Week 1 (Visit 2), Week 3 (Visit 3), and Week 5 (Visit 4))
研究者
Athanasios Koutsos
Lecturer in Human Nutrition
University of Glasgow