Adipose Tissue Gene Expression Profiles in Women With PCOS Versus Controls Matched for BMI
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Transcriptomic profiling of subcutaneous adipose tissue of women with PCOS and age and BMI matched controls
研究概览
简要总结
Adipose tissue is a central organ in mediating metabolic health. There is some evidence that women with polycystic ovary syndrome (PCOS) have a degree of adipose tissue dysfunction which may negatively affect their metabolic health. The aim of this study was to assess transcriptomic profiles of subcutaneous adipose tissue of women with PCOS in comparison with a control population matched on the basis of age and body mass index (BMI). A secondary aim was to then relate these gene expression profiles to the biochemical environment.
详细描述
Adipose tissue is a central organ in mediating metabolic health. There is some evidence that women with polycystic ovary syndrome (PCOS) have a degree of adipose tissue dysfunction which may negatively affect their metabolic health. The aim of this study was to assess transcriptomic profiles of subcutaneous adipose tissue of women with PCOS in comparison with a control population matched on the basis of age and body mass index (BMI). A secondary aim was to then relate these gene expression profiles to the biochemical environment.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Had a positive diagnosis of PCOS as defined according to the NIH criteria as chronic oligomenorrhoea (< 9 menstrual cycles per year) and clinical and/or biochemical evidence of hyperandrogenism, in the absence of other disorders causing the same phenotype. Clinical criteria included hirsutism with a Ferriman-Galwey score greater than 9, acne or male pattern alopecia; biochemical criteria included total-testosterone, androstenedione or dehydroepiandrosterone sulphate (DHEAS) greater than the laboratory reference range.
- •Were between the ages of 18 and 40
排除标准
- •Were under 18 years or greater than 40 years old,
- •Were non-Caucasian
- •Were pregnant, lactating or trying to conceive
- •Had a body mass index (BMI) <18kg/m2 or >50kg/m2
- •Had a recent illness or any chronic illness likely to influence results
- •Were taking hormonal contraception
结局指标
主要结局
Transcriptomic profiling of subcutaneous adipose tissue of women with PCOS and age and BMI matched controls
时间窗: Recruitment ran from Dec 2007-July 2008. Subjects attended the study location fasting on one occasion
Gene expression profiles from women with PCOS and controls matched for age and BMI were determined by microarray analysis of whole subcutaneous adipose tissue samples. Single gene changes as well as pathway analyses will be used to determine differently expressed genes and pathways between the PCOS cohort and controls.
次要结局
- Measurement of biomarkers of metabolic health (lipid, inflammatory markers) in plasma of PCOS cases and controls(Recruitment ran from Dec 2007-July 2008. Subjects attended the study location fasting on one occasion)