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A Phase 2, Placebo-Controlled Study To Evaluate The Efficacy And Safety Of PF-00489791 In Patients With Type 2 Diabetes And Overt Nephropathy

Phase 2
Completed
Conditions
Diabetic Nephropathies
Interventions
Drug: Placebo
Registration Number
NCT01200394
Lead Sponsor
Pfizer
Brief Summary

PF-00489791 is an inhibitor of phosphodiesterase type 5. Our hypothesis is that PF-00489791 will enhance the relaxation of blood vessels within the kidney and so reduce blood pressure, improving renal function.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
256
Inclusion Criteria
  • Male or female subjects greater than or equal to 18 years. Female subjects must be of non-child bearing potential.
  • Clinical diagnosis of type 2 diabetes together with stages 3a, 3b or 4 CKD, based on an eGFR of 25-59 mL/min/1.73m2.
  • Evidence of persistent, overt albuminuria; defined as a UACR greater than or equal to 300 mg/g (greater than or equal to 33.9 mg/mmol) for greater than 3 months.
Exclusion Criteria
  • Subjects with CKD resulting from type 1 diabetes or non-diabetic CKD.
  • Subjects with poorly controlled diabetes mellitus, defined as HbA1C >9%.
  • Subjects on combination ACE inhibitor/ARB therapy.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlacebo-
PF-00489791PF-00489791-
Primary Outcome Measures
NameTimeMethod
Change From Baseline in Urinary Albumin Creatinine Ratio (UACR) at Week 12Baseline, Week 12 (Day 5, 6, 7)

UACR was ratio of albumin measured in urine (milligram) to creatinine measured in urine (millimole), reported in units milligram per millimole (mg/mmol). A decrease in UACR may be associated with improved renal and cardiovascular function. The mean values of the 3 consecutive first morning void urine samples (obtained 2 days prior to \[Day 5, 6 of Week 12\], and with last sample collected on the morning of scheduled clinic visit \[Day 7 of Week 12\]) were used to determine UACR at the scheduled clinic visit. The mean values of the 3 consecutive first morning void urine samples obtained at screening were used to determine baseline UACR.

Secondary Outcome Measures
NameTimeMethod
Change From Baseline in Urinary Albumin Creatinine Ratio (UACR) at Week 3, 6 and 16Baseline, Week 3 (Day 5, 6, 7), Week 6 (Day 5, 6, 7), Week 16 (Day 5, 6, 7)

UACR was ratio of albumin measured in urine (milligram) to creatinine measured in urine (millimole), reported in units mg/mmol. A decrease in UACR may be associated with improved renal and cardiovascular function. The mean values of the 3 consecutive first morning void urine samples (obtained 2 days prior to \[Day 5, 6 of specified Week\], and with last sample collected on the morning of scheduled clinic visit \[Day 7 of specified Week\]) were used to determine UACR at the scheduled clinic visit. The mean values of the 3 consecutive first morning void urine samples obtained at screening were used to determine baseline UACR.

Change From Baseline in Urinary Protein Creatinine Ratio (UPCR) at Week 3, 6, 12, and 16Baseline, Week 3 (Day 5, 6, 7), Week 6 (Day 5, 6, 7), Week 12 (Day 5, 6, 7), Week 16 (Day 5, 6, 7)

UPCR is a ratio between two measured substances in urine: milligram of protein per millimole (mmol) of creatinine, reported in units mg/mmol. A decrease in UPCR may be associated with improved renal and cardiovascular function. The mean values of the 3 consecutive first morning void urine samples (obtained 2 days prior to \[Day 5, 6 of Week 3, 6, 12, 16\], and with last sample collected on the morning of scheduled clinic visit \[Day 7 of Week 3, 6, 12, 16\]) were used to determine UPCR at the scheduled clinic visit. The mean values of the 3 consecutive first morning void urine samples obtained at screening were used to determine baseline UPCR.

Change From Baseline in Serum Creatinine Concentration at Week 3, 6, 12, and 16Baseline, Week 3, 6, 12, 16 (follow-up)

Serum creatinine concentration was used as a marker of renal function. Baseline serum creatinine concentration was determined predose at Week 0 (Day 1).

Change From Baseline in Estimated Glomerular Filtration Rate (eGFR) at Week 3, 6, 12, and 16Baseline, Week 3, 6, 12, 16 (follow-up)

The eGFR was calculated using 4 variable formula developed by the modification of diet in renal disease (MDRD) study group. The 4 variables needed to estimate glomerular filtration rate (GFR) using this formula were serum creatinine concentration (sCr), age, sex (for females, eGFR was multiplied by 0.742) and ethnic origin (for African-Caribbean people only, eGFR was multiplied by 1.212). Thus eGFR in milliliter per minute per 1.73 square meter (mL/min/1.73 m\^2) = 175\*(sCr/88.4)\^-1.154\*(Age)\^-0.203\*(0.742 if female)\*(1.212 if African-Caribbean). Baseline eGFR was determined predose at Week 0 (Day 1).

Systolic, Diastolic and Mean Blood Pressure at Week 0, 3, 6, 12, and 16Week 0, 3, 6, 12, 16 (follow-up)

Systolic blood pressure (SBP) is the blood pressure (pressure exerted by circulating blood on the walls of blood vessels) when heart is contracting; it is the maximum arterial pressure during contraction of left ventricle of heart. diastolic blood pressure (DBP) is the blood pressure (pressure exerted by circulating blood on the walls of blood vessels) when heart is relaxing; it is the minimum arterial pressure during relaxation and dilation of ventricles of heart. Mean blood pressure (MBP) = diastolic blood pressure + (\[systolic blood pressure - diastolic blood pressure\]/3). After a minimum of 5 minutes of rest, supine BP was measured with the participant's arm supported at the level of the heart.

Change From Baseline in Urine Transforming Growth Factor (TGF) Beta-1 Concentration at Week 3, 6, 12, and 16Baseline, Week 3, 6, 12, 16 (follow-up)

TGF Beta-1 is a major fibrogenic growth factor implicated in the pathogenesis of renal scarring. It is overexpressed in the diabetic kidney where it may promote matrix accumulation. Baseline TGF Beta-1 concentration was determined predose at Week 0 (Day 1).

Change From Baseline in Serum High Sensitivity C-Reactive Protein (Hs-CRP) Concentration at Week 12 and 16Baseline, Week 12, 16 (follow-up)

The CRP is an acute phase reactant which is virtually absent from the blood serum of healthy persons but rapidly appears in blood and body fluids in response to injurious stimuli. Baseline hs-CRP was determined predose at Week 0 (Day 1).

Change From Baseline in Serum Cystatin-C Concentration at Week 12 and 16Baseline, Week 12, 16 (follow-up)

Cystatin C is produced by all nucleated cells at a constant rate and is freely filtered at the glomerulus. The blood concentration of cystatin C depends almost entirely on the GFR and is not substantially affected by diet, nutritional status or inflammatory disease. Serum cystatin C had been proposed as an endogenous marker of GFR in participant with chronic kidney disease (CKD) than sCr. Baseline serum cystatin C was determined predose at Week 0 (Day 1).

Plasma Concentration Versus Time Summary of PF-00489791Pre-dose at Day 1 of Week 0, 3, 6 and 12; 4 hours post-dose on Day 1 of Week 0, 3 and 6

Trial Locations

Locations (156)

North America Research Institute

🇺🇸

Azusa, California, United States

California Kidney Specialists

🇺🇸

San Dimas, California, United States

Research Across America

🇺🇸

Houston, Texas, United States

North American Research Institute / California Kidney Specialist

🇺🇸

Azusa, California, United States

Northwest Louisiana Nephrology

🇺🇸

Shreveport, Louisiana, United States

Lincoln Nephrology and Hypertension

🇺🇸

Lincoln, Nebraska, United States

Houston Nephrology Research

🇺🇸

Houston, Texas, United States

N/A - formerly with Entralogix SMO

🇨🇦

Toronto, Ontario, Canada

P. D. Hinduja National Hospital and Medical Research Centre

🇮🇳

Mumbai, Maharashtra, India

Deenanath Mangeshkar Hospital & Research Centre

🇮🇳

Pune, Maharashtra, India

Diabetes Care and Research Centre

🇮🇳

Pune, Maharashtra, India

KE.M Hospital Research Centre

🇮🇳

Pune, Maharashtra, India

Saadat Ansari Internal Medicine

🇺🇸

Huntsville, Alabama, United States

Southwest Clinical Research Institute, LLC

🇺🇸

Tempe, Arizona, United States

ASA Clinical Research, LLC

🇺🇸

Jupiter, Florida, United States

Lakeview Medical Research

🇺🇸

Summerfield, Florida, United States

Chicago Clinical Research Institute, Inc.

🇺🇸

Chicago, Illinois, United States

Four Rivers Clinical Research, Inc.

🇺🇸

Paducah, Kentucky, United States

Crescent City Clinical Research Center

🇺🇸

Metairie, Louisiana, United States

Biolab Research, LLC

🇺🇸

Rockville, Maryland, United States

Alzohaili Medical Consultants

🇺🇸

Dearborn, Michigan, United States

Apex Medical Research, AMR, Inc.

🇺🇸

Flint, Michigan, United States

Apex Medical Research, MI, Inc.

🇺🇸

Flint, Michigan, United States

Columbia Nephrology Associates, PA

🇺🇸

Columbia, South Carolina, United States

Carolina Nephrology, PA

🇺🇸

Greenville, South Carolina, United States

Palmetto Nephrology, PA

🇺🇸

Orangeburg, South Carolina, United States

South Carolina Nephrology & Hypertension Ctr, Inc

🇺🇸

Orangeburg, South Carolina, United States

South Carolina Nephrology and Hypertension Center

🇺🇸

Orangeburg, South Carolina, United States

Renal Research Practice

🇦🇺

Gosford, New South Wales, Australia

John Hunter Hospital

🇦🇺

Newcastle, New South Wales, Australia

Department of Nephrology

🇦🇺

New Lambton, Newcastle, Australia

Pharmacy Department

🇦🇺

New Lambton, Newcastle, Australia

Melbourne Renal Research Group

🇦🇺

Reservoir, Victoria, Australia

University of Alberta Hospital

🇨🇦

Edmonton, Alberta, Canada

Entralogix Clinical Research Inc.

🇨🇦

Oakville, Ontario, Canada

London Health Sciences Centre

🇨🇦

London, Ontario, Canada

Sunnybrook Health Sciences Center

🇨🇦

Toronto, Ontario, Canada

Saskatoon Nephrology Group, Nurses Redisence

🇨🇦

Saskatoon, Saskatchewan, Canada

Saskatoon Nephrology Group, Nurses Residence

🇨🇦

Saskatoon, Saskatchewan, Canada

Gujarat Kidney Foundation

🇮🇳

Ahmedabad, Gujarat, India

Apollo Hospitals

🇮🇳

Hyderabad, Andhra Pradesh, India

Pfizer Centre

🇮🇳

Mumbai, Maharashtra, India

Jehangir Clinical Development Centre Pvt. Ltd.

🇮🇳

Pune, Maharashtra, India

Pharmacy

🇰🇷

Seongnam-si, Gyeonggi-do, Korea, Republic of

Seoul National University Hospital

🇰🇷

Seoul, Korea, Republic of

Samsung Medical Center,Sungkyunkwan Univ School of Medicine

🇰🇷

Seoul, Korea, Republic of

Unit Hemodialisis, Hospital Serdang

🇲🇾

Kajang, Selangor, Malaysia

Nephrology Clinic, Queen Elizabeth Hospital

🇲🇾

Kota Kinabalu, Sabah, Malaysia

Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran (INCMNSZ)

🇲🇽

Tlalpan, Mexico CITY, Mexico

NZOZ "DIAGNOMED" S.C., Poradnia Nefrologiczna

🇵🇱

Bielsko-Biala, Poland

NZOZ PS "Medica"

🇵🇱

Lublin, Poland

Centrum Medyczne "Osteomed" NZOZ, Lecznica Specjalistow

🇵🇱

Warszawa, Poland

SPZOZ Akademicki Szpital Kliniczny im. J. Mikulicza - Radeckiego

🇵🇱

Wroclaw, Poland

Centrum Medyczne "Osteomed"

🇵🇱

Warszawa, Poland

Clinic for Nephrology, Military Medical Academy

🇷🇸

Belgrade, Serbia

Clinical Hospital Center Zvezdara

🇷🇸

Belgrade, Serbia

Clinic for Endocrinology, Clinical Center Nis

🇷🇸

Nis, Serbia

FNsP Bratislava, Nemocnica Stare Mesto

🇸🇰

Bratislava, Slovakia

Wits Clinical research

🇿🇦

Johannesburg, Gauteng- South Africa, South Africa

Nemocnice s poliklinikami n.o.

🇸🇰

Levice, Slovakia

Vseobecna nemocnica Rimavska Sobota

🇸🇰

Rimavska Sobota, Slovakia

Worthwhile Clinical Trials (WWCT), Lake View Hospital

🇿🇦

Benoni, Gauteng, South Africa

Division of Nephrology and Hypertension, E13 Renal Unit

🇿🇦

Cape Town, South Africa

Dr. George Mukhari Hospital -University of Limpopo

🇿🇦

Pretoria, Gauteng, South Africa

Centre for Diabetes and Endocrinology

🇿🇦

Houghton, Johannesburg, South Africa

Latros International

🇿🇦

Bloemfontein, South Africa

St Augustine's Hospital

🇿🇦

Durban, South Africa

Sahlgrenska University Hospital Njurmottagningen

🇸🇪

Goteborg, Sweden

Research Offices (5th Floor)

🇬🇧

Coventry, United Kingdom

Medi-Clinic Heart Hospital (Pretoria Heart Hospital)

🇿🇦

Pretoria, South Africa

A+ Science City site

🇸🇪

Stockholm, Sweden

Doncaster Royal Infirmary

🇬🇧

Doncaster, South Yorkshire, United Kingdom

The Royal London Hospital Whitechapel

🇬🇧

London, United Kingdom

Guy's and St Thomas' Foundation Trust

🇬🇧

London, United Kingdom

Northern General Hospital Campus

🇬🇧

Sheffield, United Kingdom

The Office of Iqbal Saeed MD, LLC

🇺🇸

Huntsville, Alabama, United States

Citrus Dialysis Center

🇺🇸

Covina, California, United States

California Institute of Renal Research

🇺🇸

La Mesa, California, United States

American Institute of Research

🇺🇸

Whittier, California, United States

North Valley Nephrology

🇺🇸

Yuba, California, United States

Whittier Internal Medicine Nephrology Medical Group

🇺🇸

Whittier, California, United States

Rockdale Medical Research Associates

🇺🇸

Conyers, Georgia, United States

Renal Physicians of Georgia

🇺🇸

Macon, Georgia, United States

Associates in Nephrology, SC

🇺🇸

Evergreen Park, Illinois, United States

Research by Design, LLC

🇺🇸

Evergreen Park, Illinois, United States

RenalCare Associates

🇺🇸

Peoria, Illinois, United States

Investigative Clinical Research of Indiana, LLC

🇺🇸

Elwood, Indiana, United States

Mountain Kidney and Hypertension Associates, PA

🇺🇸

Asheville, North Carolina, United States

Trial Management Associates

🇺🇸

Wilmington, North Carolina, United States

Lake Medical Research

🇺🇸

Willoughby Hills, Ohio, United States

Northeast Clinical Research Center, LLC

🇺🇸

Bethlehem, Pennsylvania, United States

Preferred Primary Care Physicians, Inc.

🇺🇸

Uniontown, Pennsylvania, United States

Diagnostic Clinic of Houston, PA

🇺🇸

Houston, Texas, United States

Nephrology Associates of Northern Virginia, Inc.

🇺🇸

Fairfax, Virginia, United States

Nephrology Specialists, P.C.

🇺🇸

Mechanicsville, Virginia, United States

Clinical Research Associates of Tidewater

🇺🇸

Norfolk, Virginia, United States

Renal Remission & Hypertension Consultants, PLLC

🇺🇸

Bremerton, Washington, United States

Sound Medical Research

🇺🇸

Port Orchard, Washington, United States

Renal Remission and Hypertension Clinic

🇺🇸

Silverdale, Washington, United States

Alliance Against Diabetes

🇺🇸

Las Vegas, Nevada, United States

Clinical Research Consortium

🇺🇸

Las Vegas, Nevada, United States

Renal Associates, PA

🇺🇸

San Antonio, Texas, United States

San Antonio Kidney Disease Center Physicians Group, P.L.L.C.

🇺🇸

San Antonio, Texas, United States

Kansas Nephrology Research Institute, LLC

🇺🇸

Wichita, Kansas, United States

Aarhus Universitetshospital (Aarhus Sygehus)

🇩🇰

Aarhus, Denmark

Rigshospitalet

🇩🇰

Copenhagen Oe, Denmark

Steno Diabetes Center

🇩🇰

Gentofte, Denmark

Akademiska Sjukhuset

🇸🇪

Uppsala, Sweden

Intercare Parow Medical and Dental Centre

🇿🇦

Parow, South Africa

Central Texas Kidney Associates

🇺🇸

Austin, Texas, United States

Capital Nephrology Clinical Research

🇺🇸

Sacramento, California, United States

SMG-SNU Boramae Medical Center

🇰🇷

Seoul, Korea, Republic of

Clinical Research Consultants, LLC

🇺🇸

Kansas City, Missouri, United States

Miedzyleski Szpital Specjalistyczny w Warszawie

🇵🇱

Warsaw, Poland

Riverside Clinical Research

🇺🇸

Edgewater, Florida, United States

Shrushrut Clinical Research Association

🇮🇳

Ahmedabad, Gujarat, India

Palm Springs Research Institute

🇺🇸

Hialeah, Florida, United States

Nebraska Nephrology Research Institute, LLC - Research Management, Inc.

🇺🇸

Lincoln, Nebraska, United States

Centre de Dialyse de Bois de Boulogne

🇨🇦

Montreal, Quebec, Canada

Hopital de Sacre Coeur de Montreal

🇨🇦

Montreal, Quebec, Canada

Boise Kidney and Hypertension Institute

🇺🇸

Meridian, Idaho, United States

Centre de sante et de services sociaux champlain-Charles-Le Moyne

🇨🇦

Greenfield Park, Quebec, Canada

Hospital Central Dr Ignacio Morones Prieto Unidad Regional de Osteoporosis

🇲🇽

San Luis Potosi, San Luis, Mexico

Sheldon M Chumir Health Centre

🇨🇦

Calgary, Alberta, Canada

Saskatoon Nephrology Group

🇨🇦

Saskatoon, Saskatchewan, Canada

Entralogix Clincal Research Inc.

🇨🇦

Brampton, Ontario, Canada

Hospital Pulau Pinang

🇲🇾

George Town, Pulau Pinang, Malaysia

Comite Mexicano para la Prevencion de la Osteoporosis, A.C.

🇲🇽

Mexico, DF, Mexico

Clinical Center of Serbia Institute for Endocrinology, Diabetes and Metabolic Diseases

🇷🇸

Belgrade, Serbia

Universiti Sains Malaysia

🇲🇾

Kota Bharu, Kelantan, Malaysia

Samsung Medical Center/Division of Nephrology

🇰🇷

Seoul, Korea, Republic of

Clinical Trial Pharmacy

🇰🇷

Seongnam-si, Korea, Republic of

Clinical Research Centre, Hospital Pulau Pinang

🇲🇾

George Town, Pulau Pinang, Malaysia

Clinical Hospital Center "Zvezdara"

🇷🇸

Belgrade, Serbia

Fakultna nemocnica s poliklinikou J.A.Reimana Presov

🇸🇰

Presov, Slovakia

Boramae Medical Center/Division of Nephrology

🇰🇷

Seoul, Korea, Republic of

AKDHC Medical Research Services, LLC*

🇺🇸

Glendale, Arizona, United States

Samodzielny Publiczny Szpital Kliniczny im Andrzeja Mieleckiego

🇵🇱

Katowice, Poland

Centrum Medyczne OSTEOMED NZOZ; Lecznica Specjalistaw

🇵🇱

Warszawa, Poland

King Edward Memorial Hospital

🇮🇳

Pune, Maharashtra, India

Samsung Medical Center, Department of Pharmacy

🇰🇷

Seoul, Korea, Republic of

Seoul National University Bundang Hospital

🇰🇷

Seongnam-si, Gyeonggi-do, Korea, Republic of

Unit Kajian Klinikal, Hospital Universiti Sains Malaysia

🇲🇾

Kubang Kerian, Kelantan, Malaysia

Hospital Angeles del Pedregal

🇲🇽

Angeles Del Pedregal Cp., Mexico

Centrum Medyczne "OSTEOMED" NZOZ

🇵🇱

Warszawa, Poland

Clinical Center of Serbia

🇷🇸

Belgrade, Serbia

ICLE SC

🇲🇽

Guadalajara, Jalisco, Mexico

Jacobi Medical Center - Department of Medicine - Nephrology

🇺🇸

Bronx, New York, United States

Research Management, Inc.

🇺🇸

Austin, Texas, United States

Hospital Taiping

🇲🇾

Taiping, Perak, Malaysia

University of Edinburgh

🇬🇧

Edinburgh, United Kingdom

Division of Nephrology, Dept. of Medicine

🇭🇰

Pokfulam, Hong Kong

Queen Mary Hospital

🇭🇰

Hong Kong, Hong Kong

ICON Clinical Research

🇭🇰

Quarry Bay, Hong Kong

Division of Nephrology

🇭🇰

Pokfulam, Hong Kong

Prince of Wales Hospital

🇭🇰

Shatin, Hong Kong

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