Implication of Genetic Variations in Long Intergenic Non-coding RNA 00511 (LINC00511) in Colorectal Cancer

1. Introduction

1.1. Background Colorectal cancer (CRC) represents the 7th most common cancer in Egypt. The global burden of CRC is expected to increase 60%, by 2030, in terms of new cases and deaths.

Traditional treatments including radio- and chemo-therapies are associated with various undesirable side-effects. Meanwhile, the 5-year survival rate for CRC is ~ 64% but drops to 12% for metastatic CRC. Therefore, development of reliable and accurate prognostic markers is necessary. Long non-coding RNAs (lncRNAs) play an important role in different types of cancer through regulation of gene expression, protein synthesis, being epigenetic signatures.

Long intergenic ncRNA 00511 (LINC00511) is a 2265 bp ncRNA that exerts an oncogenic function in many cancers, such as glioma, ovarian cancer and CRC. Single nucleotide polymorphisms (SNPs) in lncRNAs have been found to be associated with cancer. Such genetic variants may increase or reduce the risk of cancer depending on the location of these SNPs. Recently, LINC00511 SNPs were associated with breast cancer (BC) risk in Chinese population and currently, the chief supervisor is studying LINC00511 SNPs in Egyptian BC. Moreover, to address implication of LINC00511 SNPs in CRC, being not studied yet, might be useful for understanding CRC pathogenesis, linking LINC00511 SNPs to disease severity, as well as for discovering new target for CRC prevention and/or treatment.

1.2. Aim of the work Investigation of the association between LINC00511 SNPs (rs17780195 or rs9906859 and rs1558535) and CRC susceptibility and/or pathogenesis in addition to finding out the interaction between these SNPs and clinicopathological factors such as histopathological type, tumor size, lymph node metastasis and tumor grade. 2. Subjects Study Participants; will be Classified into two main groups