The Influence of Various Maternal, Infant and Environmental Factors on Human Milk Composition Among Lithuanian Women
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Breast Milk Collection
- Sponsor
- Vilnius University
- Enrollment
- 174
- Locations
- 1
- Primary Endpoint
- Quantification of total protein concentration in human milk
- Status
- Completed
- Last Updated
- 5 years ago
Overview
Brief Summary
This study aims for characterization of different maternal, infant and environmental factors (e.g. maternal age, pregnancy duration, lactation period, baby gender, circadian rhythm, Holder pasteurisation, etc.) that influence human milk composition.
Detailed Description
The study consists of three parts. In the first part the investigators aim to evaluate human milk macronutrient composition depending on the time after delivery and pregnancy duration. In the second part the investigators aim to evaluate the circadian variation of human milk macronutrient and energy content depending on pregnancy duration. In the third part the investigators aim to evaluate the influence of Holder pasteurisation on human milk macronutrient, metabolome and bioactive protein (lysozyme and lactoferrin) content.
Investigators
Eligibility Criteria
Inclusion Criteria
- •having obtained informed consent; after a single-birth pregnancy; women who were not on a special diet; lactating mothers who could not breastfeed their newborns due to the baby's medical condition (either prematurity or disease) but who expressed milk.
Exclusion Criteria
- •breastfeeding mothers; history of maternal diabetes, hepatitis B or C, HIV, tuberculosis, mastitis, or oncological disease; drug addicted.
Outcomes
Primary Outcomes
Quantification of total protein concentration in human milk
Time Frame: from the 2nd week, up to 2 months after delivery
Mid-infrared spectrophotometry
Secondary Outcomes
- Quantification of lysozyme in human milk(14-16 days after delivery)
- Quantification of lactoferrin in human milk(14-16 days after delivery)
- Quantification of total lipids and carbohydrate in human milk(from the 2nd week, up to 2 months after delivery)
- Human milk metabolome analysis(15-17 days after delivery)