Loss of Insulinotropic Properties of Glucagon-like Peptide-1 (GLP-1) and Glucose-dependent Insulinotropic Peptide (GIP) After Glucocorticoid Induced Insulin Resistance
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 19
- 试验地点
- 1
- 主要终点
- The potentiating effects of GLP-1 on second phase insulin response when clamped at at bloodsugar of 7 mmol/l
研究概览
简要总结
In this study we examine whether inducing insulin resistance by dexamethasone in healthy volunteers will lead to a decreased insulinotropic effect of the hormones GLP-1 and GIP.
详细描述
In this study we are going to examine the insulinotropic properties of GLP-1 and GIP before and after the development of insulin resistance and/or glucose intolerance.The insulinotropic properties of GLP-1 and GIP are greatly reduced in type 2 diabetes.
Since the development of type 2 diabetes is preceded by insulin resistance and glucose intolerance we wanted to examine the insulinotropic properties of GLP-1 and GIP in the early stages of type 2 diabetes.
To do this, we want to induce insulin resistance and/or glucose intolerance. This is achieved by 5 days of treatment with dexamethasone.
Subjects are studied on 4 different days in randomized order, with an oral glucose tolerance test (OGTT) and glucose clamps + infusions of GLP-1, GIP and NaCl before and immediately after 5 days treatment with dexamethasone, 2mg bid. On day 1, 2 and 3 after the 5 days of dexamethasone, 2mg dexamethasone was given in the afternoon after the tests to ensure unchanged insulin resistance
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Caucasians above 20 and below 45 years of age
- •Normal glucose tolerance as assessed by the WHO criteria
- •First degree relative and at least 1 second degree relative with type 2 diabetes
- •Normal haemoglobin
- •Informed consent
排除标准
- •Liver disease (ALAT/ASAT > 2 times normal value)
- •Kidney disease (S-creatinin > 130uM and/or albuminuria)
- •Heart disease (NYHA II, III or IV)
- •Treatment with medicine that cannot be paused
- •Pregnancy of breast feeding
研究组 & 干预措施
Dexamethasone
This is a before-after study. All subjects are administered dexamethasone 2mg BID for 5 days.
干预措施: Dexamethasone (Drug)
结局指标
主要结局
The potentiating effects of GLP-1 on second phase insulin response when clamped at at bloodsugar of 7 mmol/l
时间窗: 1-3 days after dexamethasone treatment
After dexamethasone treatment subjects are tested with infusion of GLP-1 during a clamped blood-sugar of 7mmol/l. Their increase in the second phase insulin response is the primary endpoint.
次要结局
- First phase insulin response to GLP-1 infusion at 7mmol/l(1-3 days after dexamethasone)
- Second phase insulin response to GIP infusion at 7mmol/l(1-3 days after dexamethasone treatment)
- First phase insulin response to GIP infusion at 7mmol/l(1-3 days after dexamethasone)
研究者
Carsten Dirksen
MD
Hvidovre University Hospital