Effect of a Single Bout of Moderate-intensity Aerobic Exercise on Colon Cancer Cell Growth in Vitro
试验速览
- 阶段
- 不适用
- 入组人数
- 16
- 试验地点
- 4
- 主要终点
- Cell viability (%)
研究概览
简要总结
This study involves drawing blood samples from men before and after they perform 30-minutes of moderate-intensity aerobic exercise. The investigators will evaluate whether adding the exercise serum to colon cancer cells in a dish can reduce the growth of the cells compared to the resting serum.
Note: serum is the liquid part of the blood that carries hormones and metabolites around the body.
详细描述
Regular exercise is associated with a reduced risk of developing colon cancer. However, the mechanisms underpinning the anti-cancer effect of exercise are not yet fully understood. A recent theory suggests that each time you exercise, the short-lasting spikes in circulating hormones can suppress the growth of cancer cells. Hence, every exercise bout could have a direct anti-cancer effect.
This study will recruit men with an increased of colon cancer and explore whether incubating colon cancer cells with serum collected after a bout of moderate-intensity aerobic exercise influences cell viability in vitro.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Prevention
- 盲法
- None
盲法说明
It is not possible to blind participants nor investigators. Investigators will be blind to allocation order until after the first blood sample is drawn.
入排标准
- 年龄范围
- 50 Years 至 90 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Age ≥ 50 years
- •BMI ≥ 25 kg/m2 and/or waist circumference of ≥ 94 cm
- •Participating in less than 30 min of planned, structured, moderate to vigorous-intensity physical activity on three or more d·wk-1 for the last three months
排除标准
- •Any absolute or relative contraindication to exercise testing, as determined by the American College of Sports Medicine
- •Any sign/symptom of cardiovascular, metabolic or renal disease
- •Known cardiovascular, metabolic or renal disease without written medical clearance from physician
- •Resting hypertension (≥160 mmHg systolic and/or ≥90 mmHg diastolic)
- •Chronic obstructive pulmonary disease and/or asthma with peak respiratory flow < 300 l/min
- •Previous stroke or transient ischemic attack
- •Epilepsy or aneurysm (large vessel or cerebral)
- •Previous or current treatment for malignancy
- •Clotting disorder
- •Taking beta-adrenergic blocking agents
- •Resting heart rate ≥ 100 bpm
- •Musculoskeletal, neurological, anthropometric, or rheumatoid conditions that makes it not possible to pedal a bicycle and/or would be worsened due to exercise
- •Body mass > 150 kg
结局指标
主要结局
Cell viability (%)
时间窗: 48 hours
Viability of a human colon cancer cell line (LoVo) will be assessed via quantification of the fluorescent signal by the resazurin assay (Sigma-Aldrich, Dorset, UK). Cells will be seeded in culture medium containing 10% serum from individual participants and incubated for 48 hours. Fluorescence will then be measured using a microplate reader at an excitation of 540 nm and emission of 590 nm. Background fluorescence will be subtracted from each well and then values will be normalised to fluorescence of control cells grown in 10% fetal bovine serum instead of participant serum to give a percent viability.
次要结局
- Concentration of Tumour Necrosis Factor alpha (pg/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of Interleukin 6 (pg/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of Insulin-like growth factor 1 (ng/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of epinephrine (pg/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of norepinephrine (pg/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of Insulin (pmol/l)(Immediately before and immediately after the exercise and resting assessments)