Effect of a Single Bout of Moderate-intensity Aerobic Exercise on Colon Cancer Cell Growth in Vitro
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Colon Adenocarcinoma
- Sponsor
- Northumbria University
- Enrollment
- 16
- Locations
- 2
- Primary Endpoint
- Cell viability (%)
- Last Updated
- 5 years ago
Overview
Brief Summary
This study involves drawing blood samples from men before and after they perform 30-minutes of moderate-intensity aerobic exercise. The investigators will evaluate whether adding the exercise serum to colon cancer cells in a dish can reduce the growth of the cells compared to the resting serum.
Note: serum is the liquid part of the blood that carries hormones and metabolites around the body.
Detailed Description
Regular exercise is associated with a reduced risk of developing colon cancer. However, the mechanisms underpinning the anti-cancer effect of exercise are not yet fully understood. A recent theory suggests that each time you exercise, the short-lasting spikes in circulating hormones can suppress the growth of cancer cells. Hence, every exercise bout could have a direct anti-cancer effect. This study will recruit men with an increased of colon cancer and explore whether incubating colon cancer cells with serum collected after a bout of moderate-intensity aerobic exercise influences cell viability in vitro.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Age ≥ 50 years
- •BMI ≥ 25 kg/m2 and/or waist circumference of ≥ 94 cm
- •Participating in less than 30 min of planned, structured, moderate to vigorous-intensity physical activity on three or more d·wk-1 for the last three months
Exclusion Criteria
- •Any absolute or relative contraindication to exercise testing, as determined by the American College of Sports Medicine
- •Any sign/symptom of cardiovascular, metabolic or renal disease
- •Known cardiovascular, metabolic or renal disease without written medical clearance from physician
- •Resting hypertension (≥160 mmHg systolic and/or ≥90 mmHg diastolic)
- •Chronic obstructive pulmonary disease and/or asthma with peak respiratory flow \< 300 l/min
- •Previous stroke or transient ischemic attack
- •Epilepsy or aneurysm (large vessel or cerebral)
- •Previous or current treatment for malignancy
- •Clotting disorder
- •Taking beta-adrenergic blocking agents
Outcomes
Primary Outcomes
Cell viability (%)
Time Frame: 48 hours
Viability of a human colon cancer cell line (LoVo) will be assessed via quantification of the fluorescent signal by the resazurin assay (Sigma-Aldrich, Dorset, UK). Cells will be seeded in culture medium containing 10% serum from individual participants and incubated for 48 hours. Fluorescence will then be measured using a microplate reader at an excitation of 540 nm and emission of 590 nm. Background fluorescence will be subtracted from each well and then values will be normalised to fluorescence of control cells grown in 10% fetal bovine serum instead of participant serum to give a percent viability.
Secondary Outcomes
- Concentration of Tumour Necrosis Factor alpha (pg/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of Interleukin 6 (pg/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of Insulin-like growth factor 1 (ng/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of epinephrine (pg/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of norepinephrine (pg/ml)(Immediately before and immediately after the exercise and resting assessments)
- Concentration of Insulin (pmol/l)(Immediately before and immediately after the exercise and resting assessments)