Probiotic Supplementation on Sport Performance and Gut Damage in Females

Not Applicable

Completed

• Conditions: Gut Damage; Sport Performance
• Interventions: Dietary Supplement: Placebo; Dietary Supplement: Probiotic

• Registration Number: NCT06220110
• Lead Sponsor: University of the Basque Country (UPV/EHU)

Brief Summary

This study will be performed with the aim to evaluate the effects of probiotic supplementation on sport performance and gut damage. Participants will be divided into two groups, placebo and experimental group. With the purpose to assess the effects of probiotics on the gut damage, a strenuous exercise session will be performed, and then blood samples will be taken to observe the variation of the selected intestinal damage marker. 24 hours after this strenuous session, performance tests will be performed. After the 4 weeks of supplementation, the same procedure will be followed to evaluate the effect of probiotics.

Detailed Description

One week before the initiation of the study participants will be required to avoid consuming drugs, alcohol, cigarettes, probiotics, and any other food or liquid which could modify gut microbiota composition. For the baseline values, height, weight and sports performance test, of the participants will be collected. The same day, a strenuous exercise session will be performed in order to observe the variation of the intestinal damage marker (blood sample extraction just after finishing the strenous exercise session). 24 hours after that session sport performance tests will be conducted. Participants will then be randomly divided into two groups, one of which will take one probiotic capsule per day containing 10 billion colony-forming units (CFU) of Lactobacillus plantarum, and the other group will take one placebo capsule per day consisting of dextrose. The supplementation period will last 4 weeks.

After the supplementation period, the same process will be followed, with the same strenuous exercise session and immediate blood sampling to determine the effects of probiotics on the intestinal damage caused by exercise. 24 hours later, performance tests will be conducted to assess the effects of probiotics on sport performance.

Study Timeline

• Study Start: 2023-10-15
• Primary Completion: 2023-11-15
• Study Completion: 2023-12-15
• Status Verified: 2024-01
• Study First Submitted: 2024-01-12
• Study First Submitted QC: 2024-01-12
• Study First Posted: 2024-01-23
• Last Update Submitted: 2024-01-23
• Last Update Posted: 2024-01-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 10

Inclusion Criteria

• Healthy
• No injuries
• 1 year of CrossFit practicing experience

Exclusion Criteria

• Drug, alcohol, cigarettes, probiotic intake
• Injuries

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Placebo group	Placebo	Placebo intake. Placebo capsules will contain dextrose (1 capsule per day)
Experimental group	Probiotic	Probiotic intake containing 10 billions of Colony Forming Units of Lactobacillus plantarum (1 capsule per day)

Primary Outcome Measures

Name	Time	Method
Intestinal-fatty acid-binding protein	0-10 minutes after strenuous exercise blood samples will be extracted	Gut damage marker
Counter movement jump test	24 hours after strenuous training session. First test to be performed	Jumping test
Running Based Anaerobic Sprint Test	24 hours after strenuous training session. Second test to be performed	Running test
YO YO Intermittent Recovery Level test 1	24 hours after strenuous training session. Third test to be performed	aerobic test

Trial Locations

Locations (1)

CrossFit Bikain; 🇪🇸 Vitoria, Alava, Spain