Effects of Omega-3 Fatty Acid Supplementation on Muscle Damage and Oxylipin Production Induced by Eccentric Exercise in Young Men
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 2
- 主要终点
- Maximum isometric voluntary contraction strength loss
研究概览
简要总结
In recent decades, omega-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been proposed as potential therapeutic agents for minimizing muscle damage induced by eccentric exercise, especially when supplementation is long-term (> 6 weeks) and at high doses (> 1 g/d). However, no study has analyzed whether these effects are mediated by an increase in oxylipins (metabolites derived from EPA and DHA), which play an essential role in the muscle regeneration process. The objective of the present study is to determine the effect of eight weeks of high-dose omega-3 fatty acid supplementation on blood oxylipin levels and their association with muscle function and markers of muscle damage after high-intensity eccentric exercise in young men.
详细描述
Research question Does supplementation with high doses of omega-3 for eight weeks reduce muscle damage induced by a session of high-intensity eccentric exercise in young men? Is the response to eccentric exercise related to an increase in oxylipins derived from omega-3 fatty acids? Hypothesis Long-term supplementation (8 weeks) with high doses of omega-3 (3 g/d) will increase the concentration of oxylipins in the blood, which will be associated with less muscle damage after an eccentric exercise protocol in young men.
General objective To determine the effect of eight weeks of high-dose omega-3 fatty acid supplementation on blood oxylipin levels and indirect markers of muscle damage after high-intensity eccentric exercise in young men.
Specific objectives
- To determine the effect of eight weeks of high-dose omega-3 fatty acid supplementation on blood oxylipin levels after high-intensity eccentric exercise in young men.
- To determine the effect of eight weeks of high-dose omega-3 fatty acid supplementation on muscle function and markers of muscle damage after high-intensity eccentric exercise in young men.
- Associate blood oxylipin levels with changes in muscle function and markers of muscle damage induced by a session of eccentric exercise, before and after high-dose omega-3 fatty acid supplementation in young men.
Methodology This study is an experimental, prospective, double-blind design. Sample size: The sample size calculation establishes a minimum of 12 subjects per group based on an α level of 0.05 and a power (1-β) of 0.8, assuming an estimated maximum strength loss difference of 22% after eccentric exercise (effect size = 1.06) between the omega-3 and control groups, based on a previous study (1). However, 15 participants will be recruited per group to account for a 20% dropout rate.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Investigator)
盲法说明
The masking process will ensure that both the participants and the investigators involved in the study remain unaware of the treatment assignments throughout the entire duration of the study. A unique identification code will be assigned to each participant upon enrollment, which conceals their treatment allocation. The allocation sequence will be generated by a person not directly involved in the study. The capsules in the experimental intervention and the control treatment, will be packaged identically to maintain visual similarity. The packaging materials and labeling will be designed to be indistinguishable between the two groups. In addition, all outcome assessments and data collection procedures will be performed by personnel who are blinded to the treatments
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Healthy male participants between 18 and 40 years
排除标准
- •Participants must not have performed regular strength training during the 6 months prior to the start of the study.
- •Participants must also not be taking medications that affect inflammation or cardiovascular responses to exercise and must have a BMI of less than 30 kg/m
- •Participants must not consume fish more than twice a week and must not have taken any omega-3 supplements for three months prior to the study or be allergic to fish.
结局指标
主要结局
Maximum isometric voluntary contraction strength loss
时间窗: Pre and post intervention (8 weeks)
Change in MVC strength loss 0, 2, 24, 48, and 72 hours after.
Plasma oxylipins
时间窗: Pre and post intervention (8 weeks)
Levels of plasma oxylipins
次要结局
- Peak aerobic capacity(Pre and post intervention (8 weeks))
- Dietary assessment(Pre and post intervention (8 weeks))
- Creatine kinase(Pre and post intervention (8 weeks))
- Muscle soreness(Pre and post intervention (8 weeks))
- Fatty acid composition(Pre and post intervention (8 weeks))
- Physical activity levels(Pre and post intervention (8 weeks))
- Thigh circumference(Pre and post intervention (8 weeks))
- Vertical Jump Performance(Pre and post intervention (8 weeks))
研究者
Sebastian Jannas-Vela
Associate Professor
Instituto de Ciencias de la Salud, Universidad de O'Higgins