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Clinical Performance and Reproducibility of the Accelerate PhenoTest™ Blood Culture (BC) Kit With New Antibiotic Wave I Updates

Completed
Conditions
Bacteremia
Interventions
Device: Specimens that meet inclusion criteria
Registration Number
NCT04295915
Lead Sponsor
Accelerate Diagnostics, Inc.
Brief Summary

This is a performance and reproducibility study to validate new or updated antimicrobials on the Accelerate PhenoTest™ BC kit. The data from this study will be used to support the submission to the FDA for clearance in the US and for global registrations of the device intended for in vitro diagnostic use.

Detailed Description

This clinical study entitled "Clinical Performance and Reproducibility of the Accelerate PhenoTest™ BC Kit Using the Accelerate Pheno™ System with New Antibiotic Wave I Updates for Antimicrobial Susceptibility Testing of Positive Blood Culture Specimens" is designed to demonstrate the clinical performance and reproducibility of the new or updated antimicrobials on the Accelerate PhenoTest™ BC kit, which was previously FDA-cleared in February 2017.

Clinical performance and reproducibility evaluations will be conducted at a minimum of three sites to determine the clinical performance of the new or updated antimicrobials from a combination of fresh (de-identified, remnant specimens) and contrived positive blood culture samples, to include at least 375 total specimens.

The study sites selected will have expertise in blood culture diagnostics and will be able to conduct the study in accordance with this protocol and Good Clinical Practices (GCP). Additionally, prior to study initiation, each site will have approval from the local Institutional Review Board (IRB).

It is anticipated that the data from this study will be used to support the submission to the FDA for clearance in the US and for global registrations of the device intended for in vitro diagnostic use.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
731
Inclusion Criteria
  • Blood culture positivity is ≤ 8 hours prior to initiation of testing with the Accelerate Pheno™ system
  • Positive blood culture sample containing gram-negative rods according to Gram stain results
  • For fresh specimens, de-identified, remnant positive blood culture specimens from patients for which Standard of Care (SOC) testing has been initiated and the remaining samples would otherwise be discarded
  • A minimum sample volume of 2.0 mL
  • Seeded blood culture specimens derived from archived bacterial
Exclusion Criteria
  • Blood culture positivity is > 8 hours prior to initiation of testing with the Accelerate Pheno™ system
  • Insufficient (less than 2.0 mL) remnant sample volume
  • From a patient that has already been enrolled in the study
  • Fresh samples from Mycobacterial-type blood culture media (BACTEC™ Myco/F Lytic, BacT/ALERT® MP Bottle, VersaTREK® Myco) or charcoal-containing media
  • Seeded specimens that appear mixed (by Gram stain or purity plating) after bottle culture positivity

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Specimens that meet inclusion criteriaSpecimens that meet inclusion criteria-
Primary Outcome Measures
NameTimeMethod
Essential and categorical agreement with Clinical and Laboratory Standards Institute (CLSI) broth microdilution reference methodApproximately 4 months
Secondary Outcome Measures
NameTimeMethod
Essential agreement of MICs compared to the modal MIC values obtained for intra- and inter-laboratory resultsApproximately 2 months

Trial Locations

Locations (3)

Penn State-Hershey Medical Center

🇺🇸

Hershey, Pennsylvania, United States

Quest/med fusion

🇺🇸

Lewisville, Texas, United States

Accelerate Diagnostics

🇺🇸

Tucson, Arizona, United States

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