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Migraine Induction Properties of PACAP-38 After Eptinezumab in Migraine Without Aura Patients.

Not Applicable
Completed
Conditions
Migraine
Interventions
Registration Number
NCT05635604
Lead Sponsor
Danish Headache Center
Brief Summary

To investigate whether administration of calcitonin gene-related peptide (CGRP) binding monoclonal antibodies eptinezumab prevents pituitary adenylate cyclase-activating polypeptide-38 (PACAP-38) induced migraine attacks in migraine without aura patients.

Detailed Description

The aim of the present study is to investigate whether

- CGRP binding monoclonal antibodies eptinezumab prevents PACAP-38 induced migraine attacks in migraine without aura patients.

Clinical and basic research have established calcitonin gene-related peptide (CGRP) as central molecule in migraine pathophysiology, yet existing CGRP antagonizing therapies such as the CGRP and CGRP receptor binding monoclonal antibodies are only effective in 50-60% of migraine patients. PACAP-38 and CGRP colocalize in the trigeminovascular system, and both activate adenylate cyclase upon receptor binding which causes increased formation of cyclic AMP (cAMP). It is unknown if CGRP and PACAP-38 signaling pathways differ sufficiently for PACAP-38 to be an alternative treatment target to CGRP. Whether administration of CGRP binding monoclonal antibodies eptinezumab prevents PACAP-38 induced migraine attacks in migraine without aura patients is yet to be investigated.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
38
Inclusion Criteria
  • Healthy volunteers of both sexes.
  • 18-60 years.
  • 50-100 kg.
  • Women of childbearing potential must use adequate contraception
  • History of migraine without aura for ≥ 12 months according to the International Classification of Headache Disorders 3rd Edition (ICHD-3) criteria.
  • No migraine preventive treatment.
Exclusion Criteria
  • A history of serious somatic disease
  • > 50 years of age at migraine onset.
  • History of any other primary headaches disorder (except ≤ 5 monthly days with tension- type headache).
  • Daily intake of any medication except contraceptives

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Eptinezumab and PACAP-38EptinezumabThe participants will receive an intravenous infusion of eptinezumab (300 mg) over 30 minutes followed (2 hours later) by an intravenous infusion of PACAP-38 (10 pmol/kg/min) over 20 minutes.
Eptinezumab and PACAP-38PACAP-38The participants will receive an intravenous infusion of eptinezumab (300 mg) over 30 minutes followed (2 hours later) by an intravenous infusion of PACAP-38 (10 pmol/kg/min) over 20 minutes.
Placebo and PACAP-38PACAP-38The participants will receive an intravenous infusion of placebo (saline) over 30 minutes followed (2 hours later) by an intravenous infusion of PACAP-38 (10 pmol/kg/min) over 20 minutes.
Primary Outcome Measures
NameTimeMethod
A subjective report of a migraine attackTime of reporting migraine attack is from the baseline to 24 hours after eptinezumab/placebo administration.

The participants report whether they feel a migraine attack (Yes or no).

Secondary Outcome Measures
NameTimeMethod
HeadacheTime of headache measurements is from from the baseline to 24 hours eptinezumab/placebo administration.

Headache intensity will be recorded by numerical rating scale (NRS) from 0 to 10 (0, no headache; 1, a feeling of pressure; 10, worst imaginable headache).

Changes in the superficial temporal artery (STA).Time of headache measurements is from from the baseline to 24 hours eptinezumab/placebo administration.

Diameter of the frontal branch of the superficial temporal artery (STA).

Repeated measurements covering the diameter of STA before and after eptinezumab/placebo administration and PACAP-38 infusion measured by millimeters (mm).

Trial Locations

Locations (1)

Danish headache center

🇩🇰

Glostrup, Denmark

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