Gut Microbiota Association With ESBL-E Colonisation and Subsequent ESBL-E Infection
- Conditions
- Extended Spectrum Beta-Lactamase Producing Bacteria InfectionMicrobial ColonizationCritical Illness
- Interventions
- Diagnostic Test: ESBL-E fecal carriage screening according to routine care
- Registration Number
- NCT04131569
- Lead Sponsor
- University of Bordeaux
- Brief Summary
Antimicrobial resistance is a major threat worldwide and extended-spectrum beta-lactamase producing Enterobacteriales (ESBL-E) are a leading cause because of their wide dissemination. Gut microbiota seems to be correlated with multi-drug resistant organism carriage. This study thus aims to analyse the correlation between gut microbiota, ESBL-E fecal carriage and subsequent infection.
- Detailed Description
The rising antimicrobial resistance has led to more than 33,000 deaths in Europe in 2015. Among them, extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) are the most frequent in Europe and have disseminated both in the community and in healthcare settings. Some studies have suggested that microbiota could be different between multi-drug resistant organisms, with different relative abundances of some bacteria. One study focused on ESBL-E fecal carriers, but in the community, with Bacteroides uniformis being more abundant in ESBL-E non-carriers than carriers. As identification of species discriminating between ESBL-E fecal carriers and non-carriers could pave the way for the design of ESBL-E carriage eliminating probiotics, we aim to analyse the correlation between gut microbiota and ESBL-E fecal carriage.
Moreover, mechanisms in the link between ESBL-E fecal carriage and subsequent ESBL-E infection remain, so far, poorly understood and this study aims to provide a first insight in the involvement of gut microbiota in the link between colonization and infection.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 60
- Patient above 18 year-old admitted to intensive care unit
- ESBL-E fecal carriage according to current screening recommendations for ESBL-E carriage group
- Feces quantity on rectal swab adequate for routine screening and microbiota analysis
- Guardianship, curatorship, or prisoners
- No health insurance
- No legal representative
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description ESBL-E fecal carriers ESBL-E fecal carriage screening according to routine care Patients with a positive ESBL-E fecal carriage according to routine screening non ESBL-E fecal carriers ESBL-E fecal carriage screening according to routine care Patients without positive ESBL-E fecal carriage according to routine screening
- Primary Outcome Measures
Name Time Method Gut bacteriobiota diversity according to ESBL specie at positive screening Comparison of gut bacteriobiota alpha diversity between ESBL E. coli and ESBL K. pneumoniae fecal carriers
- Secondary Outcome Measures
Name Time Method Bacteria and the absence of subsequent ESBL-E infection at admission Association of bacteria with ESBL-E subsequent infection among ESBL-E fecal carriers by LefSe method
Fungi and the absence of subsequent ESBL-E infection at admission Association of fungi with ESBL-E subsequent infection among ESBL-E fecal carriers by LefSe method
bacteria and the absence of ESBL E. coli fecal carriage at admission Association of bacteria with the absence of ESBL E. coli fecal carriage by LefSe method
Gut mycobiota and subsequent ESBL-E infection at admission Analysis of gut mycobiota beta diversity between ESBL-E fecal carriers subsequently ESBL-E infected and non-subsequently ESBL-E infected
Gut mycobiota diversity according to ESBL specie at positive screening Analysis of gut mycobiota beta diversity between ESBL E. coli and ESBL K. pneumoniae fecal carriers
Gut bacteriobiota and subsequent ESBL-E infection at admission Analysis of gut bacteriobiota beta diversity between ESBL-E fecal carriers subsequently ESBL-E infected and non-subsequently ESBL-E infected
Gut bacteriobiota diversity according to ESBL specie at positive screening Analysis of gut bacteriobiota beta diversity between ESBL E. coli and ESBL K. pneumoniae fecal carriers
fungi and the absence of ESBL E. coli fecal carriage at admission Association of fungi with the absence of ESBL E. coli fecal carriage by LefSe method
fungi and the absence of ESBL K. pneumoniae fecal carriage at admission Association of fungi with the absence of ESBL K. pneumoniae fecal carriage by LefSe method
bacteria and the absence of ESBL K. pneumoniae fecal carriage at admission Association of bacteria with the absence of ESBL K. pneumoniae fecal carriage by LefSe method
Trial Locations
- Locations (1)
Medical intensive care unit, Pelelgrin hospital
🇫🇷Bordeaux, Nouvelle-Aquitaine, France