Comparative study of the efficacy of swabbed and spit saliva for detection of red complex bacteria and salivary inflammatory markers in periodontitis
试验速览
- 阶段
- 2 期
- 状态
- 尚未招募
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Detection of red complex bacteria in swabbed and spit saliva at 4 degree Celsius and room temperature
研究概览
简要总结
To compare the efficacy of swabbed and spit saliva samples at 4°C and room temperature for the detection of red complex bacteria (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) and salivary inflammatory markers (IL6, TNF-α, NF-κB) in individuals diagnosed with periodontitis. Swabbed saliva will be collected by keeping the swab in the lingual vestibule, below the tongue, for 20 s. Spit saliva will be collected by asking the participant to expectorate into a sterile container. Saliva samples will be processed for the detection of red complex bacteria (P. gingivalis, T. forsythia, and T. denticola) & salivary inflammatory markers (IL6, TNF-α, NF-κB) using Multiplex RT-PCR test kits.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 盲法
- None
入排标准
- 年龄范围
- 18.00 Year(s) 至 65.00 Year(s)（—）
- 性别
- All
入选标准
- •Participants aged 18 years and older.
- •Subjects diagnosed with periodontitis as per the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions.
- •Subjects who have not undergone any periodontal therapy in the past six months.
排除标准
- •Participants with systemic diseases that could affect periodontal health (e.g., uncontrolled diabetes).
- •Participants currently undergoing periodontal treatment.
- •History of antibiotic use within the past three months.
结局指标
主要结局
Detection of red complex bacteria in swabbed and spit saliva at 4 degree Celsius and room temperature
时间窗: 3 months
次要结局
- To detect whether the swabbed or spit saliva is better for the detection of red complex bacteria and also to identify the suitable temperature.(3 months)
研究者
Dr Majo Ambooken
Mar Baselios Dental College