Glioma Microenvironment an Exploratory Study
概览
- 阶段
- 不适用
- 干预措施
- 未指定
- 疾病 / 适应症
- Glioma of Brain
- 发起方
- Hospital Sirio-Libanes
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- gene expression changes
- 状态
- 进行中（未招募）
- 最后更新
- 2年前
概览
简要总结
Diffuse glioms are primary brain tumors characterized by infiltrative growth and high heterogeneity, which render the disease mostly incurable. Advances in genetic analysis revealed that molecular and epigenetic alterations predict patients´s overall survival and clinical outcome. However, glioma tumorigenicity is not exclusively caused by its genetic alterations. The crosstalk between tumor cells and the surrounding microenvironment plays a crucial role in modulating glioma growth and aggressiveness. In this sense, to understand the tumor microenvironment would elucidate potential treatment alternatives. The focus will be to evaluate myeloid cells and cytokines levels.
详细描述
The proposal is to study gene expression and early epigenetic changes in myeloid cells from brain tumors and co-culture experiments. Tough RNA-seq, ATAC-seg and subsequent analysis to monitor the differential gene expression through different time points followed by stimuli. The study will compare the obtained results with sequencing data from microglia of full-fledged human tumor samples (glioblastoma and low grade gliomas). Additionally it will evaluated the immunologic characteristic of the tumor through cytokines levels analysis (TNF-α, IL-4, IL-6, IL-8, IL-10, IL-13, IFN-γ, TGF-β e IL-1ra).
研究者
Raquel Chacon Ruiz Martinez
Principal investigator, PhD
Hospital Sirio-Libanes
入排标准
入选标准
- •18 - 65 years old with brain tumor
- •Patients that will be submitted to brain tumor ressection
排除标准
- •Chronic neurodegenerative and inflamatory diseases
结局指标
主要结局
gene expression changes
时间窗: 2 years
Though RNA-seq, ATAC-seg and subsequent analysis we will be able to monitor the differential gene expression through different time points followed by stimuli.
次要结局
- cytokines expression(2 years)