Independent and Combined Effects of Short-term Sulforaphane Supplementation and Exercise on Immunometabolism in Healthy Adults: A Randomized Crossover Study
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 20
- 试验地点
- 1
研究概览
简要总结
The study consist of 3 interventional periods which will be completed by each consenting participant in a randomized, crossover fashion. The interventional periods will be separated by a minimum 1 week washout period. One of the interventions consists of 4 days of daily supplementation with the commercially available broccoli sprout extract capsules (BrocElite). Another intervention will consist of 4 days of daily supervised treadmill running. The exercise protocol consists of 4 minutes of high-intensity running running followed by a 3 minute recovery period, repeated for a total of 4 repetitions. The last intervention combines the above two and consists of 4 days of daily supplementation and daily supervised exercise training. Before and after each intervention, basic anthropometrics (weight, heart rate, and blood pressure) will be recorded, and a venous blood sample will be collected. Blood samples will be analyzed for metabolic and inflammatory markers. Participants will be asked to refrain from structured exercise, alcohol consumption, and broccoli consumption during each of the experimental periods. Before any testing, willing participants will provide informed consent, and fill out a few demographic, and lifestyle questionnaires. The study will be conducted at the University of British Columbia in Kelowna, BC, Canada.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 19 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •aged between 19 and 35 years
- •not a current smoker
- •physically active based on the Canadian Physical Activity Guidelines (150 minutes of weekly physical activity)
排除标准
- •a history of cardiometabolic diseases or respiratory diseases (e.g., chronic obstructive pulmonary disease, asthma, diabetes, coronary artery disease)
- •currently following a ketogenic diet
- •body mass index (BMI) over 30 kg/m2
- •unable to read or communicate in English
- •having received a vaccination or experienced an upper respiratory tract infection in the last 4 weeks
- •having donated more than 0.5 L of blood within the last 4 weeks
- •currently pregnant
- •have contraindications to high intensity exercise (assessed using the CSEP Get Active Questionnaire)
研究组 & 干预措施
Sulforaphane Supplementation Only
In this intervention, participants will consume commercially available broccoli sprout extract capsules daily for 4 days (20 mg/day) . Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period.
干预措施: Sulforaphane-rich Broccoli Sprout Extract (Dietary Supplement)
Exercise Only
In this intervention, participants will complete supervised exercise sessions daily for 4 days. The exercise intervention consists of interval running on a treadmill which contains 4 cycles of: 4 minutes of intense running (85-95% HRmax) followed by 3 minutes at a slow, easy pace. Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period.
干预措施: High Intensity Interval Treadmill Running (Other)
Sulforaphane Supplementation and Exercise
In this intervention, participants will consume commercially available broccoli sprout extract tablets daily for 4 days. In addition, participants will complete supervised exercise sessions daily for 4 days. The exercise intervention consists of interval running on a treadmill which contains 4 cycles of: 4 minutes of intense running (85-95% HRmax) followed by 3 minutes at a slow, easy pace. Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period.
干预措施: Sulforaphane-rich Broccoli Sprout Extract (Dietary Supplement)
Sulforaphane Supplementation and Exercise
In this intervention, participants will consume commercially available broccoli sprout extract tablets daily for 4 days. In addition, participants will complete supervised exercise sessions daily for 4 days. The exercise intervention consists of interval running on a treadmill which contains 4 cycles of: 4 minutes of intense running (85-95% HRmax) followed by 3 minutes at a slow, easy pace. Basic anthropometrics (weight, heart rate, blood pressure) and a venous blood sample (30 mL) will be collected before and after this interventional period.
干预措施: High Intensity Interval Treadmill Running (Other)
结局指标
主要结局
未指定
次要结局
- Change in percentage of classical monocytes before and after the 6-week intervention(0-4 days)
- Change in percentage of intermediate monocytes before and after the 4-day intervention(0-4 days)
- Change in percentage of non-classical monocytes before and after the 4-day intervention(0-4 days)
研究者
Hashim Islam
Assistant Professor
University of British Columbia