Panoramic Presentation and Tolerance Mechanism of Human Endometrium Based on Tiangui Theory
- Conditions
- Endometrium; Senility
- Interventions
- Procedure: Endometrial biopsy
- Registration Number
- NCT04860310
- Lead Sponsor
- Heilongjiang University of Chinese Medicine
- Brief Summary
Detailed Description:In the process of women's menstrual cycle, there are different physiology at different time points in a cycle.
Change, investigators select women with a menstrual cycle of 28 days and take samples every 3 days.
Day 4, Day 7, Day 10, Day 13, Day 16, Day 19, Day 22, Day 25, Day 28 days, a total of 10 time points, 20 healthy women were selected, and the transcription, Protein and phosphorylation proteomics, systematically integrated analysis; at the same time, 20 subjects were classified by age group Divide into 4 groups, 21-year-old group, 28-year-old group, 35-year-old group, and 42-year-old group. At the same time, investigators then analyze the differences in the receptivity of the endometrium of different ages.
The experimental design groups are: 21-year-old group, 28-year-old group, 35-year-old group, 42-year-old group, Each group includes D1 group Vs D4 group Vs D7 group Vs D10 group Vs D13 group Vs D16 group Vs D19 group Vs D22 group Vs D25 group Vs D28 group; in phosphorylation modification omics, different time points are compared with each other, Look for transcripts, proteins and phosphorylated proteins that change significantly during the cycle of change. Analyze phosphorylation modification An important role for changing proteins and an important role for cycle changes.
The experiment quantified the proteome and protein acylation modification through TMT (Tandem Mass Tag), and passed GO, KEGG Pathway, Localization, Functional enrichment analysis, Cluster analysis Bioinformatics analysis
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- 55
Not provided
Not provided
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description 35 years old group Endometrial biopsy - 42 years old group Endometrial biopsy - 21 years old group Endometrial biopsy - 28 years old group Endometrial biopsy - 49 years old group Endometrial biopsy -
- Primary Outcome Measures
Name Time Method Panorama of the endometrium Menstrual cramps 24 hours, the seventh day of menstruation, LH peak, 72 hours after the peak, the 21st day of menstruation, the 28th day of menstruation Discovery of inner membrane receptive proteins and pathways
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Department of Obstetrics and Gynecology,First Affliated Hospital,Heilongjiang University of Chinese Medicine .
🇨🇳Harbin, Heilongjiang, China