MedPath

Anakinra in Dengue With Hyperinflammation ( AnaDen )

Phase 2
Recruiting
Conditions
Hyperinflammatory Syndrome
Macrophage Activation Syndrome
Severe Dengue
Anti-inflammatory Agents
Dengue
Dengue With Warning Signs
Anakinra
Immuno-modulation
Cytokine Storm
Interventions
Drug: Placebo
Registration Number
NCT05611710
Lead Sponsor
Oxford University Clinical Research Unit, Vietnam
Brief Summary

This study aims to evaluate the effect of anakinra in dengue patients with hyperinflammation as compared to placebo

Primary Objective:

To evaluate the efficacy of Anakinra in moderate-severe dengue patients with hyperinflammation.

Secondary Objectives:

* To assess the safety of anakinra therapy in dengue with hyperinflammation

* To assess the effect of anakinra therapy in patients with dengue on physiological, clinical and virological parameters

* To assess the immunomodulation effects of anakinra in dengue

* Immune cell signatures in dengue with and without anakinra

* To assess difference in gene expression between treatment group compared to non-treatment population

Detailed Description

This is a randomized double blinded placebo controlled trial investigating the effects of four days of anakinra treatment on dengue patients with hyperinflammatory syndrome. The anakinra/placebo will be given to eligible participants admitted to the Hospital for Tropical Diseases (HTD) in Ho Chi Minh City, Vietnam. 160 dengue patients will be randomly assigned to either anakinra or placebo intervention group to receive treatment for 4 days.

Patients admitted to the HTD with a clinical diagnosis of dengue and at least 1 warning sign(s) or severe dengue to Emergency department / inpatient wards / Intensive Care Units (ICU), will be invited to participate in the trial.

Eligible patients will be invited to participate in the screening phase during which, the collection of clinical information about this acute illness episode as well as some screening tests will be performed, including measurement ferritin, creatinine, pregnancy test (for all females).

- If ferritin level is greater than 2000ng/mL and meet all other inclusion/exclusion criteria, patients will be invited to participate in the randomization phase (second consent), which they will be randomly given either anakinra or placebo intravenous (IV) for four days.

The intervention:

* (i) 200mg bid for four days in adults participants (≥ 16 years) or in children (12-16 years), with weight \> 50kg; and

* (ii) 2mg/kg bid for four days in children (12-16 years), with weight \< 50Kg.

All patients will be followed up daily at the clinical wards until discharge.

Details of all AEs and SAEs will be recorded on specific forms, together with an assessment as to whether the events are likely to have been related to any treatment received. All SAEs will be reported promptly to the DMC and ECs according to policy. In cases of discontinuation due to AEs, participants will be followed up until the events have resolved or stabilized.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
160
Inclusion Criteria
  • Patients hospitalised with a clinical diagnosis of dengue and at least 1 warning sign(s) (see appendix) or severe dengue to Emergency department/inpatient wards/Intensive Care wards (ICU),
  • Ferritin levels > 2000ng/mL
  • ≥ 12 years of age
  • Written informed consent or assent to participate in the study
  • Agree to come back for 2 follow up visits around day 30 of illness (maximum 5 weeks) and at 3 months
Exclusion Criteria
  • Pregnancy
  • Localizing features suggesting an alternative/additional diagnosis, e.g. pneumonia, sepsis
  • Patients taking immunosuppressive drugs or other biologics in last 1 month
  • Patients with underlying malignancy or immunosuppression
  • Children <12 years
  • Have end-stage renal failure (baseline GFR < 30ml/min)
  • Being treated for TB
  • Taking any drug with significant interaction with anakinra
  • The study physician judges that the patient is unlikely to attend follow up visit at around 3-4 weeks after fever onset - e.g. due to long travelling distance from the clinic

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboThe control group will be formed of 80 dengue patients with warning signs or severe dengue receiving placebo.
AnakinraAnakinraThe intervention group will include 80 dengue patients with warning signs or severe dengue receiving anakinra.
Primary Outcome Measures
NameTimeMethod
Change in modified Sequential Organ Failure Assessment score (mSOFA core, modified for limited resource settings and dengue) within 4 daysbaseline, up to day 4

Change in mSOFA score over 4 days after randomization (min score= 0, max score = 24, higher scores mean worse outcomes)

Secondary Outcome Measures
NameTimeMethod
MortalityUp to day 30

Number of death up to day 30

Change in Platelets countUp to day 5, at day 30

Change in blood levels (Platelets) over 5 days following randomization and at day 30

Change of ALT levelsUp to day 5, at day 30

Change in blood levels (ALT) over 5 days following randomization and at day 30

Change of Ferritin levelsUp to day 5, at day 30

Change in blood levels (Ferritin) over 5 days following randomization and at day 30

Fever clearance timeUp to day 30

Time to temperature \<37.5 for at least 48 hours

Number of Adverse Events (AEs) per participantUp to day 30

Number of AEs per individual

Change of CRP levelsUp to day 5, at day 30

Change in blood levels (CRP) over 5 days following randomization and at day 30

Area under the curve (AUC) of the serial viral load measurements during hospital stayat discharge (assessed up to day 8)

AUC of viral load measurements during hospital stay will be reported

Number of days treated in hospitalUp to day 30

Number of days treated in hospital

Number of participants with Serious Adverse Events (SAEs)Day 1-5 and Day 6-30

Number of participants having SAEs within 2 time-periods, 1- 5 days and 6-30 days

Change in neutrophils countUp to day 5, at day 30

Change in blood levels (neutrophils) over 5 days following randomization and at day 30

Time to normalization of blood levelsUp to day 30

Time to normalization of platelets (defined as \>150 x109/l) and neutrophils (\>2 x109/l)

Duration of viraemiaUp to day 30

Number of days from enrollment to the first undetectable viraemia (negative in qPCR and NS1)

Number of days treated in Intensive care unit (ICU)Up to day 30

Number of days treated in ICU

Platelet nadirUp to day 30

Lowest platelet count recorded during admission

Change in modified Sequential Organ Failure Assessment score (mSOFA core, modified for limited resource settings and dengue) at day 7baseline, day 7

Change in mSOFA score at day 7 post randomization (min score= 0, max score = 24, higher scores mean worse outcomes)

Patients' quality of life questionnaire scoreat discharge (assessed up to day 8) and at day 30

Patients' quality of life during their hospitalisation will be explored at discharge and day 30 using the EQ-5D questionnaire.

Trial Locations

Locations (1)

Hospital for Tropical Diseases

🇻🇳

Ho Chi Minh, Vietnam

© Copyright 2025. All Rights Reserved by MedPath