Oral hydroxycitric acid (HCA) supplementation enhances glycogen synthesis in exercised human skeletal 1 muscle

Completed

• Conditions: Nutritional, Metabolic, Endocrine; Glycogen synthesis in human skeletal muscle; Glycogen synthesis

• Registration Number: ISRCTN45782014
• Lead Sponsor: ational Science Council (Taiwan)

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2011-01-21
• Last Update Posted: 13 January 2015

Recruitment & Eligibility

• Status: Completed
• Sex: Male
• Target Recruitment: 8

Inclusion Criteria

1. Healthy male volunteers
2. Aged 22.0 +/- 0.3 years
3. Height 171.3 +/- 0.7 cm
4. Weight 73.7 +/- 1.1 kg
5. Body mass index (BMI) 25.2 +/- 0.5 kg/m2
6. Ventilatory equivalent for oxygen (VEO2) 108
7. Peak 45.7 +/- 1.5 ml/kg/min

Exclusion Criteria

Male volunteer's VO2max less than 30 ml/kg/min

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Glycogen assay. Approximately 25 mg of skeletal muscle from the deep portion of the vastus lateralis was dissolved in 1 N KOH at 75°C for 30 minutes. Dissolved homogenate was neutralised by glacial acetic acid and incubated overnight in acetate buffer (0.3 M sodium acetate, pH to 4.8) containing amyloglucosidase (Boehringer Mannheim, Indianapolis, IN). The reaction mixture was neutralised with 1 N NaOH. Samples were then analysed by measuring glucosyl units by the Trinder reaction (Sigma, St. Louis, MO).

Secondary Outcome Measures

Name	Time	Method
Molecular expressions of GLUT4, FAT/CD36, measured with reverse transcription-polymerase chain reaction (RT-PCR) and western blotting