Microbiome Analysis of Skin and Airway Samples and Effect of Face Masks

• Conditions: Healthy

• Registration Number: NCT04894422
• Lead Sponsor: University Hospital, Antwerp

Brief Summary

The goal of this study is to optimize two methods for microbiome analysis of skin and upper airway samples (16S amplicon sequencing and Shotgun sequencing). In addition, the researchers will investigate whether there is an effect of wearing a face mask on the microbial communities of the skin and upper airways. The volunteers will self-collect swabs of the skin and upper airways (nose and/or throat). Bacterial DNA and/or total RNA will be isolated and the microbial communities will be investigated with 16S amplicon sequencing and shotgun metagenomic sequencing.

Detailed Description

Not available

Study Timeline

• Study Start: 2021-03-15
• Status Verified: 2021-05
• Study First Submitted: 2021-05-11
• Study First Submitted QC: 2021-05-19
• Last Update Submitted: 2021-05-19
• Study First Posted: 2021-05-20
• Last Update Posted: 2021-05-20
• Primary Completion: 2021-12-31
• Study Completion: 2023-03-15

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Adult age 18 at the start of the study

Exclusion Criteria

• Pregnant women

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Optimalization of microbiome analysis of skin and upper airway samples	Start	16S rRNA sequencing, shotgun metagenomic sequencing
Change from baseline in microbiome composition of the skin and upper airways after wearing a face mask	Start, 4 hours	16S rRNA sequencing

Trial Locations

Locations (1)

University of Antwerp and University Hospital Antwerp; 🇧🇪 Antwerp, Belgium