Accelerating Post-exercise Muscle Glycogen Resynthesis in Humans: Impact of Combined Nutrient Intake and Intermittent Pneumatic Compression or Heat Therapy
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- PepsiCo Global R&D
- Enrollment
- 26
- Locations
- 1
- Primary Endpoint
- Rate of glycogen resynthesis
Study Overview
Brief Summary
To determine if modalities designed to improve blood flow combined with post-exercise nutrient intake will improve replenishment of muscle glycogen better than nutrient intake alone. A secondary objective is to compare the effectiveness of heat therapy and intermittent pneumatic compression on glycogen replenishment.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Subjects will be male and female participants in good health
- •18-35 years of age
- •Currently performing cardiovascular or intermittent exercise at a moderate to high intensity at least 5 days per week for 60 minutes or longer per day.
- •Understanding of the procedures to be undertaken as part of the study
- •Willingness to participate in exercise testing and follow instructions provided by the experimenter
- •Informed, voluntary, written consent to participate in the study
Exclusion Criteria
- •History of deep vein thrombosis
- •Obesity (BMI > 30 kg/m2)
- •Hypertension (resting SBP > 140/90 mmHg)
- •Pregnant, planning to become pregnant, or lactating
- •Supplements containing antioxidants including vitamin C and E and N-acetyl cysteine
- •Any medication
- •Lactose intolerance
- •Participation in another study within the previous 30 days or in a PepsiCo funded study within the past 6 months.
Arms & Interventions
Heat therapy
Custom water-circulating garment on leg
Intervention: Glycogen-depleting exercise session (Other)
Intermittent pneumatic compression
IPC sleeve device on leg
Intervention: Compression sleeve (Device)
Intermittent pneumatic compression
IPC sleeve device on leg
Intervention: Glycogen-depleting exercise session (Other)
Intermittent pneumatic compression
IPC sleeve device on leg
Intervention: Nutrient Intake (beverage) (Other)
Intermittent pneumatic compression
IPC sleeve device on leg
Intervention: Skeletal muscle biopsy (Procedure)
Heat therapy
Custom water-circulating garment on leg
Intervention: Heat therapy (Device)
Heat therapy
Custom water-circulating garment on leg
Intervention: Nutrient Intake (beverage) (Other)
Heat therapy
Custom water-circulating garment on leg
Intervention: Skeletal muscle biopsy (Procedure)
Outcomes
Primary Outcomes
Rate of glycogen resynthesis
Time Frame: Change from baseline to 1.5 hrs and 3.5 hrs post exercise bout. Biopsy 1 post-exercise pre nutrient intake 1 and compression (IPC) or heat, biopsy 2 post compression or heat, pre-nutrition intake 2, biopsy 3 at 210 min. Higher rate is better.
Leg skeletal muscle glycogen level assessment using assay kit
Muscle functional recovery: Skeletal muscle function
Time Frame: Change from pre-exercise to post-exercise and prior to biopsies and treatments, and 24 hours post exercise. Less fatigue is better.
Work fatigue (%) of the knee extensors measured using an isokinetic dynamometer
Muscle functional recovery: Perceived muscle soreness
Time Frame: Change from pre-exercise to post-exercise and prior to biopsies and treatments, and 24 hours post exercise. Less soreness is better.
Knee extensor muscle soreness on a visual analog scale (VAS) of 100 mm after stepping on and off a 40 cm (female) or 45 cm (male) box three times
Secondary Outcomes
- Serum Glucose(Change from post exercise at same time as biopsy 1 (Time 0), biopsy 2 (1.5 hrs) and biopsy 3 (3.5 hrs)
- mRNA expression of genes involved in regulation of glucose and glycogen metabolism and muscle functional recovery(Change from baseline to 1.5 hours and 3.5 hours post exercise bout. Biopsy 1 post-exercise pre- nutrient intake 1 and compression (IPC) or heat, biopsy 2 post compression or heat and pre- nutrition intake 2, biopsy 3 at 210 min. Higher expression better.)
- Serum insulin(Change from post exercise at same time as biopsy 1 (Time 0), biopsy 2 (1.5 hrs) and biopsy 3 (3.5 hrs))