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The Effect of Doxycycline on Matrix Metalloproteinase Expression and Activity in the Abdominal Aneurysm

Phase 2
Completed
Conditions
Aortic Aneurysm, Abdominal
Interventions
Registration Number
NCT00538967
Lead Sponsor
Leiden University Medical Center
Brief Summary

The matrix metalloproteinase-9 (MMP-9) is considered to play a central role in abdominal aortic aneurysm (AAA) initiation. Doxycycline has direct MMP-9 inhibiting properties in vitro, and it effectively suppresses AAA development in rodents. Observed inhibition of AAA progression, and contradictory findings in human studies evaluating the effect of doxycycline therapy on aortic wall MMP-9 suggest that the effects of doxycycline extend beyond MMP-9 inhibition, and that the effect may be dose dependent.

Detailed Description

In this clinical trial, we evaluated the effect of two weeks of low (50 mg/day), medium (100 mg/day) or high dose (300 mg/day) doxycycline versus no medication in patients undergoing elective AAA repair. The effect of doxycycline treatment on MMP and cysteine proteases, and their respective inhibitors was evaluated in an integrative approach.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
60
Inclusion Criteria
  • Elective open aneurysm repair
Exclusion Criteria
  • Severe kidney and liver dysfunction

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
4doxycyclinedoxycycline 300 mg
2doxycyclinedoxycycline 50 mg/day
3doxycyclinedoxycycline 100 mg
Primary Outcome Measures
NameTimeMethod
Collagenase mRNA and protein expression2 weeks
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Leiden University Medical Center

🇳🇱

Leiden, Netherlands

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